Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1056878..1057380 Replicon chromosome
Accession NZ_CP055015
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HUZ78_RS05520 Protein ID WP_000767829.1
Coordinates 1056878..1057132 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HUZ78_RS05525 Protein ID WP_001259255.1
Coordinates 1057129..1057380 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS05490 (1051893) 1051893..1052120 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
HUZ78_RS05495 (1052134) 1052134..1053192 - 1059 WP_000492346.1 thiosulfate utilization transporter TsuA/YeeE -
HUZ78_RS05500 (1053371) 1053371..1054729 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HUZ78_RS05505 (1054719) 1054719..1054781 - 63 WP_010723108.1 membrane protein YoeI -
HUZ78_RS05510 (1054996) 1054996..1055925 - 930 WP_032191639.1 LysR substrate-binding domain-containing protein -
HUZ78_RS05515 (1055971) 1055971..1056795 - 825 WP_000754737.1 SDR family oxidoreductase -
HUZ78_RS05520 (1056878) 1056878..1057132 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HUZ78_RS05525 (1057129) 1057129..1057380 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HUZ78_RS05530 (1057663) 1057663..1057713 + 51 WP_001364200.1 his operon leader peptide -
HUZ78_RS05535 (1057859) 1057859..1058758 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HUZ78_RS05540 (1058764) 1058764..1060068 + 1305 WP_033560786.1 histidinol dehydrogenase -
HUZ78_RS05545 (1060065) 1060065..1061135 + 1071 WP_000108972.1 histidinol-phosphate transaminase -
HUZ78_RS05550 (1061135) 1061135..1062202 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T160991 WP_000767829.1 NZ_CP055015:c1057132-1056878 [Shigella flexneri]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T160991 NZ_CP070986:c2127324-2127115 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTAATTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT160991 WP_001259255.1 NZ_CP055015:c1057380-1057129 [Shigella flexneri]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT160991 NZ_CP070986:c2127629-2127336 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCGATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References