Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 342734..343320 Replicon chromosome
Accession NZ_CP055003
Organism Klebsiella pneumoniae strain STIN_79

Toxin (Protein)


Gene name doc Uniprot ID A0A331ME34
Locus tag HUZ54_RS01600 Protein ID WP_025861226.1
Coordinates 342952..343320 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag HUZ54_RS01595 Protein ID WP_004174006.1
Coordinates 342734..342955 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ54_RS01575 (HUZ54_01565) 338890..339816 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HUZ54_RS01580 (HUZ54_01570) 339813..341090 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
HUZ54_RS01585 (HUZ54_01575) 341087..341854 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HUZ54_RS01590 (HUZ54_01580) 341856..342569 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HUZ54_RS01595 (HUZ54_01585) 342734..342955 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUZ54_RS01600 (HUZ54_01590) 342952..343320 + 369 WP_025861226.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HUZ54_RS01605 (HUZ54_01595) 343593..344909 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HUZ54_RS01610 (HUZ54_01600) 345016..345903 + 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HUZ54_RS01615 (HUZ54_01605) 345900..346745 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HUZ54_RS01620 (HUZ54_01610) 346747..347817 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 338890..348554 9664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13551.98 Da        Isoelectric Point: 8.6410

>T160914 WP_025861226.1 NZ_CP055003:342952-343320 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGIKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T160914 NZ_CP070963:c122965-122690 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT160914 WP_004174006.1 NZ_CP055003:342734-342955 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT160914 NZ_CP070963:c123249-122965 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331ME34

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References