Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3725205..3725746 Replicon chromosome
Accession NZ_CP055000
Organism Shigella flexneri strain STIN_80

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag HUZ55_RS18575 Protein ID WP_000615979.1
Coordinates 3725468..3725746 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ55_RS18570 Protein ID WP_000729704.1
Coordinates 3725205..3725465 (+) Length 87 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ55_RS18550 (3720888) 3720888..3721673 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
HUZ55_RS18555 (3721645) 3721645..3723357 + 1713 Protein_3650 flagellar biosynthesis protein FlhA -
HUZ55_RS18560 (3723573) 3723573..3724070 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
HUZ55_RS18565 (3724246) 3724246..3725019 - 774 WP_001353765.1 C40 family peptidase -
HUZ55_RS18570 (3725205) 3725205..3725465 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ55_RS18575 (3725468) 3725468..3725746 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ55_RS18580 (3725902) 3725902..3726642 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ55_RS18585 (3726613) 3726613..3727380 - 768 WP_000333379.1 class II glutamine amidotransferase -
HUZ55_RS18590 (3727586) 3727586..3728164 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3665253..3750460 85207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-91)

Antitoxin

(4-84)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T160900 WP_000615979.1 NZ_CP055000:3725468-3725746 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T160900 NZ_CP070962:2300966-2301073 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT160900 WP_000729704.1 NZ_CP055000:3725205-3725465 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT160900 NZ_CP070962:c2300919-2300853 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1FHQ9


Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References