Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3719016..3719710 Replicon chromosome
Accession NZ_CP055000
Organism Shigella flexneri strain STIN_80

Toxin (Protein)


Gene name yafO Uniprot ID -
Locus tag HUZ55_RS18535 Protein ID WP_089578406.1
Coordinates 3719016..3719414 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag HUZ55_RS18540 Protein ID WP_000554757.1
Coordinates 3719417..3719710 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3714676) 3714676..3714756 - 81 NuclAT_12 - -
- (3714676) 3714676..3714756 - 81 NuclAT_12 - -
- (3714676) 3714676..3714756 - 81 NuclAT_12 - -
- (3714676) 3714676..3714756 - 81 NuclAT_12 - -
HUZ55_RS18505 (3714016) 3714016..3715260 - 1245 WP_000189541.1 esterase FrsA -
HUZ55_RS18510 (3715352) 3715352..3715810 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HUZ55_RS18515 (3716071) 3716071..3717528 + 1458 WP_096037973.1 cytosol nonspecific dipeptidase -
HUZ55_RS18520 (3717585) 3717585..3718106 - 522 Protein_3643 peptide chain release factor H -
HUZ55_RS18525 (3718105) 3718105..3718308 - 204 Protein_3644 RtcB family protein -
HUZ55_RS18530 (3718554) 3718554..3719006 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
HUZ55_RS18535 (3719016) 3719016..3719414 - 399 WP_089578406.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HUZ55_RS18540 (3719417) 3719417..3719710 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HUZ55_RS18545 (3719762) 3719762..3720817 - 1056 WP_001226164.1 DNA polymerase IV -
HUZ55_RS18550 (3720888) 3720888..3721673 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
HUZ55_RS18555 (3721645) 3721645..3723357 + 1713 Protein_3650 flagellar biosynthesis protein FlhA -
HUZ55_RS18560 (3723573) 3723573..3724070 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3665253..3750460 85207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15506.85 Da        Isoelectric Point: 8.0949

>T160899 WP_089578406.1 NZ_CP055000:c3719414-3719016 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHFVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T160899 NZ_CP070962:2300431-2300538 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT160899 WP_000554757.1 NZ_CP055000:c3719710-3719417 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT160899 NZ_CP070962:c2300384-2300318 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References