Detailed information of TA system TA160854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3722578..3723119
• Replicon: chromosome
• Accession: NZ_CP054992
• Organism: Shigella flexneri strain STIN_87

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q47149
• Locus tag: HUZ57_RS18185
• Protein ID: WP_000615983.1
• Coordinates: 3722841..3723119 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Q47150
• Locus tag: HUZ57_RS18180
• Protein ID: WP_000729703.1
• Coordinates: 3722578..3722838 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ57_RS18160 (3718253)	3718253..3719038	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
HUZ57_RS18165 (3719010)	3719010..3720722	+	1713	Protein_3572	flagellar biosynthesis protein FlhA	-
HUZ57_RS18170 (3720946)	3720946..3721443	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
HUZ57_RS18175 (3721619)	3721619..3722368	-	750	WP_000056849.1	C40 family peptidase	-
HUZ57_RS18180 (3722578)	3722578..3722838	+	261	WP_000729703.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
HUZ57_RS18185 (3722841)	3722841..3723119	+	279	WP_000615983.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
HUZ57_RS18190 (3723275)	3723275..3724015	+	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
HUZ57_RS18195 (3723986)	3723986..3724753	-	768	WP_000333380.1	class II glutamine amidotransferase	-
HUZ57_RS18200 (3724959)	3724959..3725537	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI	3640968..3738087	97119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T160854 WP_000615983.1 NZ_CP054992:3722841-3723119 [Shigella flexneri]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T160854 NZ_CP070954:2332296-2332574 [Escherichia fergusonii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTATTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGTGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT160854 WP_000729703.1 NZ_CP054992:3722578-3722838 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT160854 NZ_CP070954:2332574-2332858 [Escherichia fergusonii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCCGCGTTGACGCCAGAAATGGCGA
TTAAACTTTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTACAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Structures

Toxin

• PDB: 4Q2U

Antitoxin

• AlphaFold DB: A0A5F1F716