Detailed information of TA system TA160853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3716381..3717075
• Replicon: chromosome
• Accession: NZ_CP054992
• Organism: Shigella flexneri strain STIN_87

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: HUZ57_RS18145
• Protein ID: WP_001263489.1
• Coordinates: 3716381..3716779 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HUZ57_RS18150
• Protein ID: WP_000554758.1
• Coordinates: 3716782..3717075 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3711969)	3711969..3712049	-	81	NuclAT_11	-	-
- (3711969)	3711969..3712049	-	81	NuclAT_11	-	-
- (3711969)	3711969..3712049	-	81	NuclAT_11	-	-
- (3711969)	3711969..3712049	-	81	NuclAT_11	-	-
HUZ57_RS18120 (3712645)	3712645..3713103	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HUZ57_RS18125 (3713364)	3713364..3714821	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
HUZ57_RS18130 (3714878)	3714878..3715399	-	522	Protein_3565	peptide chain release factor H	-
HUZ57_RS18135 (3715395)	3715395..3715601	-	207	Protein_3566	RtcB family protein	-
HUZ57_RS18140 (3715919)	3715919..3716371	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
HUZ57_RS18145 (3716381)	3716381..3716779	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HUZ57_RS18150 (3716782)	3716782..3717075	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HUZ57_RS18155 (3717127)	3717127..3718182	-	1056	WP_001226164.1	DNA polymerase IV	-
HUZ57_RS18160 (3718253)	3718253..3719038	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
HUZ57_RS18165 (3719010)	3719010..3720722	+	1713	Protein_3572	flagellar biosynthesis protein FlhA	-
HUZ57_RS18170 (3720946)	3720946..3721443	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI	3640968..3738087	97119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T160853 WP_001263489.1 NZ_CP054992:c3716779-3716381 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T160853 NZ_CP070954:1770072-1770326 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACGGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT160853 WP_000554758.1 NZ_CP054992:c3717075-3716782 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT160853 NZ_CP070954:1769824-1770075 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3