Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 606489..607288 Replicon chromosome
Accession NZ_CP054992
Organism Shigella flexneri strain STIN_87

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag HUZ57_RS02935 Protein ID WP_000347275.1
Coordinates 606489..606953 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ57_RS02940 Protein ID WP_001307405.1
Coordinates 606953..607288 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ57_RS02905 (601490) 601490..601924 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ57_RS02910 (601942) 601942..602820 - 879 WP_039268471.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ57_RS02915 (602810) 602810..603589 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ57_RS02920 (603600) 603600..604073 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ57_RS02925 (604096) 604096..605376 - 1281 WP_039268470.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ57_RS02930 (605625) 605625..606434 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ57_RS02935 (606489) 606489..606953 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ57_RS02940 (606953) 606953..607288 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ57_RS02945 (607437) 607437..609008 - 1572 WP_001273752.1 galactarate dehydratase -
HUZ57_RS02950 (609383) 609383..610717 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUZ57_RS02955 (610733) 610733..611503 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T160833 WP_000347275.1 NZ_CP054992:c606953-606489 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T160833 NZ_CP070951:1346632-1347030 [Escherichia fergusonii]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCCGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATTTATGGGGCAGAAAAAAGCCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGCTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCCGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCTGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT160833 WP_001307405.1 NZ_CP054992:c607288-606953 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT160833 NZ_CP070951:1346405-1346632 [Escherichia fergusonii]
ATGGAAACCACTGTATTTTTAAGTAATCGCAGTCAGGCTGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGACGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References