Detailed information of TA system TA160810

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 123718..123972
• Replicon: plasmid unnamed3
• Accession: NZ_CP054980
• Organism: Shigella flexneri strain STIN_92

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUZ60_RS26050
• Protein ID: WP_001312851.1
• Coordinates: 123823..123972 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	123718..123779 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ60_RS26035 (121671)	121671..122414	+	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-
HUZ60_RS26040 (122469)	122469..123029	+	561	WP_000139310.1	fertility inhibition protein FinO	-
HUZ60_RS26045 (123164)	123164..123376	+	213	WP_001541896.1	ANR family transcriptional regulator	-
- (123718)	123718..123779	-	62	NuclAT_1	-	Antitoxin
- (123718)	123718..123779	-	62	NuclAT_1	-	Antitoxin
- (123718)	123718..123779	-	62	NuclAT_1	-	Antitoxin
- (123718)	123718..123779	-	62	NuclAT_1	-	Antitoxin
HUZ60_RS26050 (123823)	123823..123972	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HUZ60_RS26055 (124240)	124240..124497	+	258	WP_001541895.1	replication regulatory protein RepA	-
HUZ60_RS26060 (124600)	124600..124783	+	184	Protein_146	plasmid copy control protein CopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / sul3	-	1..124795	124795

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T160810 WP_001312851.1 NZ_CP054980:123823-123972 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T160810 NZ_CP070930:661751-662065 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCCTAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT160810 NZ_CP054980:c123779-123718 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin