Detailed information of TA system TA160807

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88403..88672
• Replicon: plasmid unnamed3
• Accession: NZ_CP054980
• Organism: Shigella flexneri strain STIN_92

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUZ60_RS25855
• Protein ID: WP_001372321.1
• Coordinates: 88547..88672 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	88403..88468 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ60_RS25805	83477..83704	+	228	WP_071961421.1	hypothetical protein	-
HUZ60_RS25810	83697..84032	+	336	WP_014106969.1	hypothetical protein	-
HUZ60_RS25815	83945..84151	+	207	WP_000275853.1	hypothetical protein	-
HUZ60_RS25820	84177..84716	+	540	WP_032332913.1	single-stranded DNA-binding protein	-
HUZ60_RS25825	84773..85006	+	234	WP_000005987.1	DUF905 family protein	-
HUZ60_RS25830	85071..87029	+	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
HUZ60_RS25835	87084..87518	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HUZ60_RS25840	87515..88234	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
HUZ60_RS25845	88246..88434	-	189	WP_001299721.1	hypothetical protein	-
-	88246..88470	+	225	NuclAT_0	-	-
-	88246..88470	+	225	NuclAT_0	-	-
-	88246..88470	+	225	NuclAT_0	-	-
-	88246..88470	+	225	NuclAT_0	-	-
-	88403..88468	+	66	-	-	Antitoxin
HUZ60_RS25850	88456..88605	+	150	Protein_104	plasmid maintenance protein Mok	-
HUZ60_RS25855	88547..88672	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUZ60_RS25860	88892..89122	+	231	WP_001426396.1	hypothetical protein	-
HUZ60_RS25865	89120..89293	-	174	Protein_107	hypothetical protein	-
HUZ60_RS25870	89363..89569	+	207	WP_000547968.1	hypothetical protein	-
HUZ60_RS25875	89594..89881	+	288	WP_000107535.1	hypothetical protein	-
HUZ60_RS25880	90004..90825	+	822	WP_001234469.1	DUF932 domain-containing protein	-
HUZ60_RS25885	91122..91724	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HUZ60_RS25890	92045..92428	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUZ60_RS25895	92615..93304	+	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / sul3	-	1..124795	124795

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T160807 WP_001372321.1 NZ_CP054980:88547-88672 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T160807 NZ_CP070924:8899-9147 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT160807 NZ_CP054980:88403-88468 [Shigella flexneri]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin