Detailed information of TA system TA160800

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 109433..109854
• Replicon: plasmid unnamed2
• Accession: NZ_CP054979
• Organism: Shigella flexneri strain STIN_92

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUZ60_RS25080
• Protein ID: WP_096937776.1
• Coordinates: 109433..109558 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	109656..109854 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ60_RS25040 (104498)	104498..104713	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
HUZ60_RS25045 (104849)	104849..105496	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
HUZ60_RS25050 (105688)	105688..106071	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUZ60_RS25055 (106423)	106423..107013	+	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
HUZ60_RS25060 (107308)	107308..108129	-	822	WP_001234475.1	DUF932 domain-containing protein	-
HUZ60_RS25065 (108248)	108248..108535	-	288	WP_000107546.1	hypothetical protein	-
HUZ60_RS25070 (108560)	108560..108766	-	207	WP_000547965.1	hypothetical protein	-
HUZ60_RS25075 (108836)	108836..109132	+	297	Protein_115	hypothetical protein	-
HUZ60_RS25080 (109433)	109433..109558	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUZ60_RS25085 (109500)	109500..109649	-	150	Protein_117	DUF5431 family protein	-
- (109656)	109656..109854	-	199	NuclAT_0	-	Antitoxin
- (109656)	109656..109854	-	199	NuclAT_0	-	Antitoxin
- (109656)	109656..109854	-	199	NuclAT_0	-	Antitoxin
- (109656)	109656..109854	-	199	NuclAT_0	-	Antitoxin
HUZ60_RS25090 (109823)	109823..110585	-	763	Protein_118	plasmid SOS inhibition protein A	-
HUZ60_RS25095 (110582)	110582..111016	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
HUZ60_RS25100 (111071)	111071..113029	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
HUZ60_RS25105 (113095)	113095..113328	-	234	WP_000005990.1	DUF905 family protein	-
HUZ60_RS25110 (113385)	113385..113924	-	540	WP_000290801.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..157162	157162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T160800 WP_096937776.1 NZ_CP054979:c109558-109433 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T160800 NZ_CP070921:c63176-62895 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT160800 NZ_CP054979:c109854-109656 [Shigella flexneri]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin