Detailed information of TA system TA160797

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71279..71533
• Replicon: plasmid unnamed2
• Accession: NZ_CP054979
• Organism: Shigella flexneri strain STIN_92

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HUZ60_RS24850
• Protein ID: WP_239636836.1
• Coordinates: 71279..71428 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	71472..71533 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ60_RS24820 (66561)	66561..69566	+	3006	WP_001143760.1	Tn3-like element Tn3 family transposase	-
HUZ60_RS24825 (69599)	69599..69964	-	366	Protein_65	plasmid replication initiator RepA	-
HUZ60_RS24830 (69957)	69957..70439	-	483	WP_001273588.1	hypothetical protein	-
HUZ60_RS24835 (70432)	70432..70479	-	48	WP_229471593.1	hypothetical protein	-
HUZ60_RS24840 (70470)	70470..70721	+	252	WP_223195197.1	replication protein RepA	-
HUZ60_RS24845 (70738)	70738..70995	-	258	WP_000083845.1	replication regulatory protein RepA	-
HUZ60_RS24850 (71279)	71279..71428	-	150	WP_239636836.1	Hok/Gef family protein	Toxin
- (71472)	71472..71533	+	62	NuclAT_1	-	Antitoxin
- (71472)	71472..71533	+	62	NuclAT_1	-	Antitoxin
- (71472)	71472..71533	+	62	NuclAT_1	-	Antitoxin
- (71472)	71472..71533	+	62	NuclAT_1	-	Antitoxin
HUZ60_RS24855 (71789)	71789..71863	-	75	Protein_71	endonuclease	-
HUZ60_RS24860 (72109)	72109..72321	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HUZ60_RS24865 (72457)	72457..73017	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HUZ60_RS24870 (73120)	73120..73980	-	861	WP_000704513.1	alpha/beta hydrolase	-
HUZ60_RS24875 (74039)	74039..74785	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..157162	157162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5602.70 Da
• Isoelectric Point: 9.1783

>T160797 WP_239636836.1 NZ_CP054979:c71428-71279 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLYELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T160797 NZ_CP070920:c4146572-4145625 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TAGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAACTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGATCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCAGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCTGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGCGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACCAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGCTAGCCATCCTGAATGCTTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT160797 NZ_CP054979:71472-71533 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin