Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3785405..3786099 Replicon chromosome
Accession NZ_CP054977
Organism Shigella flexneri strain STIN_92

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HUZ60_RS18575 Protein ID WP_001263489.1
Coordinates 3785405..3785803 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HUZ60_RS18580 Protein ID WP_000554758.1
Coordinates 3785806..3786099 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3780993) 3780993..3781073 - 81 NuclAT_11 - -
- (3780993) 3780993..3781073 - 81 NuclAT_11 - -
- (3780993) 3780993..3781073 - 81 NuclAT_11 - -
- (3780993) 3780993..3781073 - 81 NuclAT_11 - -
HUZ60_RS18550 (3781669) 3781669..3782127 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HUZ60_RS18555 (3782388) 3782388..3783845 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HUZ60_RS18560 (3783902) 3783902..3784423 - 522 Protein_3652 peptide chain release factor H -
HUZ60_RS18565 (3784419) 3784419..3784625 - 207 Protein_3653 RtcB family protein -
HUZ60_RS18570 (3784943) 3784943..3785395 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
HUZ60_RS18575 (3785405) 3785405..3785803 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HUZ60_RS18580 (3785806) 3785806..3786099 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HUZ60_RS18585 (3786151) 3786151..3787206 - 1056 WP_001226164.1 DNA polymerase IV -
HUZ60_RS18590 (3787277) 3787277..3788062 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HUZ60_RS18595 (3788034) 3788034..3789746 + 1713 Protein_3659 flagellar biosynthesis protein FlhA -
HUZ60_RS18600 (3789970) 3789970..3790467 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T160792 WP_001263489.1 NZ_CP054977:c3785803-3785405 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T160792 NZ_CP070920:1997119-1997222 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT160792 WP_000554758.1 NZ_CP054977:c3786099-3785806 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT160792 NZ_CP070920:c1997260-1996989 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References