Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 648251..648978 Replicon chromosome
Accession NZ_CP054977
Organism Shigella flexneri strain STIN_92

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HUZ60_RS03165 Protein ID WP_000550189.1
Coordinates 648251..648565 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUZ60_RS03170 Protein ID WP_000560266.1
Coordinates 648562..648978 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ60_RS03145 (644418) 644418..645404 - 987 WP_023281494.1 Gfo/Idh/MocA family oxidoreductase -
HUZ60_RS03150 (645483) 645483..646166 - 684 WP_001183042.1 vancomycin high temperature exclusion protein -
HUZ60_RS03155 (646243) 646243..646746 - 504 WP_001295542.1 M48 family metallopeptidase -
HUZ60_RS03160 (646831) 646831..647967 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HUZ60_RS03165 (648251) 648251..648565 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HUZ60_RS03170 (648562) 648562..648978 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HUZ60_RS03175 (649023) 649023..651041 - 2019 WP_049253702.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HUZ60_RS03180 (651467) 651467..653818 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T160774 WP_000550189.1 NZ_CP054977:648251-648565 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T160774 NZ_CP070914:2600746-2600853 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAATCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT160774 WP_000560266.1 NZ_CP054977:648562-648978 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT160774 NZ_CP070914:c2600699-2600633 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References