Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3851842..3852439 Replicon chromosome
Accession NZ_CP054972
Organism Klebsiella pneumoniae strain STIN_93

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HUZ61_RS18860 Protein ID WP_004142563.1
Coordinates 3852122..3852439 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HUZ61_RS18855 Protein ID WP_004142561.1
Coordinates 3851842..3852129 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ61_RS18825 (3847922) 3847922..3848170 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HUZ61_RS18830 (3848188) 3848188..3848529 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HUZ61_RS18835 (3848560) 3848560..3849675 - 1116 WP_239593318.1 MBL fold metallo-hydrolase -
HUZ61_RS18840 (3849855) 3849855..3850436 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
HUZ61_RS18845 (3850436) 3850436..3850804 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HUZ61_RS18850 (3850924) 3850924..3851577 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HUZ61_RS18855 (3851842) 3851842..3852129 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HUZ61_RS18860 (3852122) 3852122..3852439 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ61_RS18865 (3852624) 3852624..3853667 - 1044 WP_016946853.1 DUF2157 domain-containing protein -
HUZ61_RS18870 (3854333) 3854333..3855199 - 867 WP_239593320.1 helix-turn-helix transcriptional regulator -
HUZ61_RS18875 (3855308) 3855308..3856735 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T160763 WP_004142563.1 NZ_CP054972:c3852439-3852122 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T160763 NZ_CP070914:c49553-49179 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT160763 WP_004142561.1 NZ_CP054972:c3852129-3851842 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT160763 NZ_CP070914:c50011-49643 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References