160730

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 330797..331383 Replicon chromosome
Accession NZ_CP054967
Organism Klebsiella pneumoniae strain STIN_94

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag HUZ62_RS01530 Protein ID WP_002920800.1
Coordinates 331015..331383 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag HUZ62_RS01525 Protein ID WP_004174006.1
Coordinates 330797..331018 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ62_RS01505 (326954) 326954..327880 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HUZ62_RS01510 (327877) 327877..329154 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
HUZ62_RS01515 (329151) 329151..329918 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HUZ62_RS01520 (329920) 329920..330633 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HUZ62_RS01525 (330797) 330797..331018 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUZ62_RS01530 (331015) 331015..331383 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HUZ62_RS01535 (331656) 331656..332972 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HUZ62_RS01540 (333079) 333079..333966 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HUZ62_RS01545 (333963) 333963..334808 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HUZ62_RS01550 (334810) 334810..335880 + 1071 WP_032433652.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 327877..336617 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T160730 WP_002920800.1 NZ_CP054967:331015-331383 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T160730 NZ_CP070902:2029983-2030086 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT160730 WP_004174006.1 NZ_CP054967:330797-331018 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT160730 NZ_CP070902:c2030124-2029853 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTAGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References