Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3860392..3861086 Replicon chromosome
Accession NZ_CP054965
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag HUZ63_RS18690 Protein ID WP_001263501.1
Coordinates 3860392..3860790 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag HUZ63_RS18695 Protein ID WP_000554755.1
Coordinates 3860793..3861086 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS18665 (3855757) 3855757..3856215 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
HUZ63_RS18670 (3856476) 3856476..3857933 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
HUZ63_RS18675 (3857990) 3857990..3858604 - 615 WP_000602123.1 peptide chain release factor H -
HUZ63_RS18680 (3858601) 3858601..3859740 - 1140 WP_000521578.1 RNA ligase RtcB family protein -
HUZ63_RS18685 (3859930) 3859930..3860382 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
HUZ63_RS18690 (3860392) 3860392..3860790 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HUZ63_RS18695 (3860793) 3860793..3861086 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HUZ63_RS18700 (3861138) 3861138..3862193 - 1056 Protein_3671 DNA polymerase IV -
HUZ63_RS18705 (3862264) 3862264..3863187 - 924 WP_001232551.1 putative lateral flagellar export/assembly protein LafU -
HUZ63_RS18710 (3863190) 3863190..3864053 - 864 Protein_3673 flagellar motor stator protein MotA -
HUZ63_RS18715 (3864066) 3864066..3864785 - 720 WP_000938737.1 FliA/WhiG family RNA polymerase sigma factor -
HUZ63_RS18720 (3864805) 3864805..3865272 - 468 WP_000725264.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T160718 WP_001263501.1 NZ_CP054965:c3860790-3860392 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T160718 NZ_CP070899:c60579-60097 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT160718 WP_000554755.1 NZ_CP054965:c3861086-3860793 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT160718 NZ_CP070899:c60833-60567 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References