Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 610739..611538 Replicon chromosome
Accession NZ_CP054965
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HUZ63_RS03025 Protein ID WP_000347251.1
Coordinates 610739..611203 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag HUZ63_RS03030 Protein ID WP_001296435.1
Coordinates 611203..611538 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS02995 (605740) 605740..606174 - 435 WP_000948834.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ63_RS03000 (606192) 606192..607070 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ63_RS03005 (607060) 607060..607839 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ63_RS03010 (607850) 607850..608323 - 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ63_RS03015 (608346) 608346..609626 - 1281 WP_000681930.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ63_RS03020 (609875) 609875..610684 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ63_RS03025 (610739) 610739..611203 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ63_RS03030 (611203) 611203..611538 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ63_RS03035 (611687) 611687..613258 - 1572 WP_001273939.1 galactarate dehydratase -
HUZ63_RS03040 (613633) 613633..614967 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
HUZ63_RS03045 (614983) 614983..615753 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T160700 WP_000347251.1 NZ_CP054965:c611203-610739 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T160700 NZ_CP070890:c5285468-5285085 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT160700 WP_001296435.1 NZ_CP054965:c611538-611203 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT160700 NZ_CP070890:c5285710-5285468 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References