Detailed information of TA system TA160679

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29753..30174
• Replicon: plasmid pMS6192A-NDM
• Accession: NZ_CP054941
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: MS6192_RS23755
• Protein ID: WP_001302184.1
• Coordinates: 30016..30174 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	29753..29951 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS23720	25449..26843	+	1395	WP_001037799.1	carbohydrate porin	-
MS6192_RS23725	27089..27193	-	105	Protein_27	thiosulfate reductase cytochrome B subunit	-
MS6192_RS23730	27250..27943	+	694	Protein_28	IS1 family transposase	-
MS6192_RS23735	27959..28582	+	624	WP_000912556.1	potassium channel family protein	-
MS6192_RS23740	28667..28918	+	252	Protein_30	iron-containing alcohol dehydrogenase	-
MS6192_RS23745	29207..29524	-	318	Protein_31	IS110 family transposase	-
MS6192_RS23750	29679..29784	+	106	Protein_32	plasmid SOS inhibition protein A	-
-	29753..29951	+	199	NuclAT_0	-	Antitoxin
-	29753..29951	+	199	NuclAT_0	-	Antitoxin
-	29753..29951	+	199	NuclAT_0	-	Antitoxin
-	29753..29951	+	199	NuclAT_0	-	Antitoxin
MS6192_RS23755	30016..30174	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
MS6192_RS23760	30842..31048	+	207	WP_000275854.1	hypothetical protein	-
MS6192_RS23765	31073..31360	+	288	WP_000107242.1	hypothetical protein	-
MS6192_RS23770	31479..32300	+	822	WP_001234465.1	DUF945 domain-containing protein	-
MS6192_RS23775	32596..33168	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
MS6192_RS23780	33538..33921	+	384	WP_001063020.1	relaxosome protein TraM	-
MS6192_RS23785	34112..34759	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
MS6192_RS23790	34895..35110	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaNDM-1 / sul1 / qacE / aadA2 / dfrA12	-	1..142890	142890
	-	flank	IS/Tn	-	-	27566..27943	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T160679 WP_001302184.1 NZ_CP054941:30016-30174 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T160679 NZ_CP070873:1795328-1795618 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGAAAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTGATCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT160679 NZ_CP054941:29753-29951 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin