Detailed information of TA system TA160674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4263070..4263672
• Replicon: chromosome
• Accession: NZ_CP054940
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MS6192_RS20675
• Protein ID: WP_000897305.1
• Coordinates: 4263070..4263381 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS6192_RS20680
• Protein ID: WP_000356397.1
• Coordinates: 4263382..4263672 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS20650	4258984..4259583	+	600	WP_001295269.1	glucose-1-phosphatase	-
MS6192_RS20655	4259577..4260449	+	873	WP_000920762.1	virulence factor BrkB family protein	-
MS6192_RS20660	4260446..4260883	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MS6192_RS20665	4260928..4261869	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MS6192_RS20670	4261933..4262841	-	909	WP_001162704.1	alpha/beta hydrolase	-
MS6192_RS20675	4263070..4263381	+	312	WP_000897305.1	hypothetical protein	Toxin
MS6192_RS20680	4263382..4263672	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
MS6192_RS20685	4264258..4264476	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
MS6192_RS20690	4264695..4264937	+	243	WP_001087409.1	hypothetical protein	-
MS6192_RS20695	4265267..4266196	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
MS6192_RS20700	4266193..4266828	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MS6192_RS20705	4266825..4267727	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T160674 WP_000897305.1 NZ_CP054940:4263070-4263381 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T160674 NZ_CP070867:85567-85962 [Acinetobacter johnsonii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTCATCAACAATAAGCCTCAACAAGTTTTTGAGCGCTTTAAGCA
ATATCAACTCGGCCAGCTTGCGATTTCGAGTATCACGGCATCTGAGCTGGCTTTTGGTGTAGAGAAGTCTGGATCTGAAC
GCAATAAAAAGGCGTTAAACAAGTTTTTGTCACCTTTAGAGATTCTTCCTTACGATGAACAGGCGATTTGGCATTACGCC
AAATTACGCCAGAATCTTCAATCTACAGGTAAACCGATTGGCAGCTTAGATATGCTGATCGCTGCTCATGCTTTGGCATT
GGATGTTGTATTAGTTACGAACAATACAAAAGAATTTGAACGAATTGATGGTCTGAAGCTAGAGAATTGGGTCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT160674 WP_000356397.1 NZ_CP054940:4263382-4263672 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT160674 NZ_CP070867:85340-85570 [Acinetobacter johnsonii]
ATGGAAATTGCGAAAGTATTTCAAACTGGTCGCAGTCAGGCTGTTCGCTTGCCTAAAGCCTTTCGCTTTAATGGCACGGA
AGTGGCCATCAAAAGCTTCGGTCGTGGTGTATTACTCATGCCCATCGACAATCCTTGGGATGTCATGTTAGAAGCAATAA
ATGAGTTTGAGGTTGGATTCCAATTGGAGCGAGCTGATCAAGGCGAACAGCTCCGTGAGGAGTTCAGATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7