Detailed information of TA system TA160672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3471927..3472726
• Replicon: chromosome
• Accession: NZ_CP054940
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: MS6192_RS16875
• Protein ID: WP_000347266.1
• Coordinates: 3472262..3472726 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: MS6192_RS16870
• Protein ID: WP_001307405.1
• Coordinates: 3471927..3472262 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS16855	3467712..3468482	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
MS6192_RS16860	3468498..3469832	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
MS6192_RS16865	3470207..3471778	+	1572	WP_001273741.1	galactarate dehydratase	-
MS6192_RS16870	3471927..3472262	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
MS6192_RS16875	3472262..3472726	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
MS6192_RS16880	3472781..3473590	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
MS6192_RS16885	3473839..3475119	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
MS6192_RS16890	3475142..3475615	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
MS6192_RS16895	3475626..3476405	+	780	WP_174190366.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
MS6192_RS16900	3476395..3477273	+	879	WP_001315856.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
MS6192_RS16905	3477291..3477725	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3461271..3472726	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T160672 WP_000347266.1 NZ_CP054940:3472262-3472726 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T160672 NZ_CP070865:c4231913-4231383 [Shewanella putrefaciens]
GTGAGTTATTCCAAAATTTTCAAAGAATTGGATAAATCACTACACGATAGAGTATCATTTGACTGTGGCGAAGCAGAGTT
AAACGATTTTATCCAAACTCAAGCAGCAAAACATATGCAAGCAGGTATTAGCCGTACAATGGTTTTACCTGCTGCTATGC
CATTGCCCAATCAAAAATATCCAATTTGCTCATTTTATACTATCGCACCAAGTTCAATTTGCCGTGATACCTTGCCGCAA
GCTATTGGCAAAAAGTTACCACGCTATCCCATTCCGGTCTTTCTGCTTGCTCAACTTGCGGTTCATAAAGAGTTTCATGG
CAGTGGGTTAGGTAAAGCTAGCTTAATCAAAGCACTAGAATATCTGTGGGAAATTAACGCTCACATGAGAGCTTATGCCA
TTGTTGTCGATTGCTTAACTGACCAAGCTGAATCATTCTACGCAAAATATGGTTTCGAGGTTCTTTGTGAAATCAACGGT
CGTGTCAGAATGTTTATTCCAATGAAAACAGTCGGTCAGTTATTCACTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT160672 WP_001307405.1 NZ_CP054940:3471927-3472262 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT160672 NZ_CP070865:c4232179-4231910 [Shewanella putrefaciens]
ATGGCGACTGCAAGACTTGATATCCGTTTGGATGAAGAAATCAAAGCTAAGGCTGAGAAAGCATCCGCTTTGCTCGGGTT
AAAAAGCTTAACTGAATACGTTGTTCGCTTAATGGACGAAGATGCAACTCAGGTGATAGCTGAGCATGAAAGCGTTACGG
TTAAAGCAGATGTATTCGACCAATTCATAATGGCTTGTGATGAGGCTAAGGTTCCGAATAAGGCATTGCGTGAAGCGGCT
GCATTTACTAAAAATGGTGAGTTTAAGTGA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7