Detailed information of TA system TA160653

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1519494..1519865
• Replicon: chromosome
• Accession: NZ_CP054940
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: MS6192_RS07345
• Protein ID: WP_001317028.1
• Coordinates: 1519671..1519865 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1519494..1519672 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS07320	1515449..1516822	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
MS6192_RS07325	1516951..1517886	-	936	WP_001153728.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
MS6192_RS07330	1517938..1519173	-	1236	WP_000040852.1	site-specific integrase	-
MS6192_RS07335	1519175..1519390	-	216	WP_000079604.1	excisionase XisR	-
MS6192_RS07340	1519469..1519678	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1519494..1519672	+	179	NuclAT_0	-	Antitoxin
-	1519494..1519672	+	179	NuclAT_0	-	Antitoxin
-	1519494..1519672	+	179	NuclAT_0	-	Antitoxin
-	1519494..1519672	+	179	NuclAT_0	-	Antitoxin
MS6192_RS07345	1519671..1519865	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
MS6192_RS07350	1519949..1520731	-	783	WP_060615110.1	recombination protein RecT	-
MS6192_RS07355	1520724..1523324	-	2601	WP_000105127.1	exodeoxyribonuclease VIII	-
MS6192_RS07360	1523426..1523701	-	276	WP_000632297.1	protein RacC	-
MS6192_RS07365	1523776..1523946	-	171	WP_001352098.1	conserved protein, Rac prophage	-
MS6192_RS07370	1523946..1524167	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1517938..1528461	10523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T160653 WP_001317028.1 NZ_CP054940:c1519865-1519671 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T160653 NZ_CP070602:4657180-4657282 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 179 bp

>AT160653 NZ_CP054940:1519494-1519672 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin