Detailed information of TA system TA160643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 260850..261391
• Replicon: chromosome
• Accession: NZ_CP054940
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: E9TC49
• Locus tag: MS6192_RS01185
• Protein ID: WP_000615982.1
• Coordinates: 260850..261128 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: S1EWQ0
• Locus tag: MS6192_RS01190
• Protein ID: WP_000729704.1
• Coordinates: 261131..261391 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS01170	258432..259010	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
MS6192_RS01175	259216..259983	+	768	WP_000333380.1	class II glutamine amidotransferase	-
MS6192_RS01180	259954..260694	-	741	WP_001225679.1	murein L,D-transpeptidase	-
MS6192_RS01185	260850..261128	-	279	WP_000615982.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
MS6192_RS01190	261131..261391	-	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
MS6192_RS01195	261577..262350	+	774	WP_000543899.1	C40 family peptidase	-
MS6192_RS01200	262407..262763	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
MS6192_RS01205	262756..263034	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
MS6192_RS01210	263139..265232	-	2094	WP_001140806.1	flagellar biosynthesis protein FlhA	-
MS6192_RS01215	265216..266355	-	1140	WP_000785662.1	flagellar type III secretion system protein FlhB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10816.59 Da
• Isoelectric Point: 10.0702

>T160643 WP_000615982.1 NZ_CP054940:c261128-260850 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T160643 NZ_CP070601:c508-242 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.77 Da
• Isoelectric Point: 5.0758

>AT160643 WP_000729704.1 NZ_CP054940:c261391-261131 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT160643 NZ_CP070601:c716-492 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6Z6