Detailed information of TA system TA160642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3380879..3381579
• Replicon: chromosome
• Accession: NZ_CP054939
• Organism: Streptomyces sp. NA02536

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A7H8J8H1
• Locus tag: HUT14_RS15120
• Protein ID: WP_040906891.1
• Coordinates: 3380879..3381277 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: HUT14_RS15125
• Protein ID: WP_121546429.1
• Coordinates: 3381274..3381579 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT14_RS15105	3376700..3378298	-	1599	WP_176117558.1	acyl-CoA carboxylase subunit beta	-
HUT14_RS15110	3378295..3379971	-	1677	WP_176117559.1	DUF1446 domain-containing protein	-
HUT14_RS15115	3379968..3380762	-	795	WP_176117560.1	TIGR03084 family protein	-
HUT14_RS15120	3380879..3381277	+	399	WP_040906891.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUT14_RS15125	3381274..3381579	+	306	WP_121546429.1	helix-turn-helix transcriptional regulator	Antitoxin
HUT14_RS15130	3381655..3382635	-	981	WP_176117561.1	EamA family transporter	-
HUT14_RS15135	3382895..3383347	+	453	WP_121546431.1	NUDIX domain-containing protein	-
HUT14_RS15140	3383379..3386240	-	2862	WP_176117562.1	FAD-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-115)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15370.56 Da
• Isoelectric Point: 9.8000

>T160642 WP_040906891.1 NZ_CP054939:3380879-3381277 [Streptomyces sp. NA02536]
VAEPYELRFFEDVLDWIKTLAKEDPESHLHVIAALERLQEVGPALRRPTVGAIERSRFRNMRELRPRNGGTVSIRMLFVF
DPERRAIFLVAGNKATGRRWASWYPRAIKEADDKYTAYLEALEQEKKEGQGR

Nucleotide

• Length: 399 bp

>T160642 NZ_CP070599:73637-74053 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10854.41 Da
• Isoelectric Point: 6.4671

>AT160642 WP_121546429.1 NZ_CP054939:3381274-3381579 [Streptomyces sp. NA02536]
MNLGSMDDLTADITPEKRERIDAIKAEMIDAERGHELAALRKAQGFTQVQVAKAMGVTQGRVSQIERGTARLDTSTMAAY
LHAIGGELTITATVGNLSVRL

Nucleotide

• Length: 306 bp

>AT160642 NZ_CP070599:73410-73640 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7H8J8H1