Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3308389..3309098 Replicon chromosome
Accession NZ_CP054932
Organism Actinomadura sp. NAK00032

Toxin (Protein)


Gene name HigB2 Uniprot ID -
Locus tag HUT06_RS15515 Protein ID WP_176196385.1
Coordinates 3308389..3308766 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUT06_RS15520 Protein ID WP_138639714.1
Coordinates 3308763..3309098 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUT06_RS15495 3303811..3305649 + 1839 WP_176196381.1 hydantoinase B/oxoprolinase family protein -
HUT06_RS15500 3305626..3306684 - 1059 WP_176196382.1 hypothetical protein -
HUT06_RS15505 3306734..3307429 - 696 WP_176196383.1 sel1 repeat family protein -
HUT06_RS15510 3307512..3308324 - 813 WP_176196384.1 class I SAM-dependent methyltransferase -
HUT06_RS15515 3308389..3308766 + 378 WP_176196385.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUT06_RS15520 3308763..3309098 + 336 WP_138639714.1 helix-turn-helix domain-containing protein Antitoxin
HUT06_RS15525 3309149..3309715 - 567 WP_176201384.1 hypothetical protein -
HUT06_RS15530 3309775..3310932 + 1158 WP_176196386.1 CoA transferase -
HUT06_RS15535 3310929..3312329 + 1401 WP_176196387.1 acyl-CoA synthetase -
HUT06_RS15540 3312326..3312901 - 576 WP_176196388.1 TetR/AcrR family transcriptional regulator -
HUT06_RS15545 3312978..3313619 + 642 WP_176196389.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-110)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-87)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14336.12 Da        Isoelectric Point: 4.5673

>T160637 WP_176196385.1 NZ_CP054932:3308389-3308766 [Actinomadura sp. NAK00032]
VDEEWEIRVTNEFLIWIGELEERSKAQVVDAVDRLAEDGPGLGRPLVDRLEGSEIHNLKELRPGSAGRSEIRILFVFDPW
RSAILLVGGDKSGDWSGWYRRAIPRAEELYVDYLKEREGKEGLSR

Download         Length: 378 bp

>T160637 NZ_CP070598:4075257-4075475 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12133.63 Da        Isoelectric Point: 8.5250

>AT160637 WP_138639714.1 NZ_CP054932:3308763-3309098 [Actinomadura sp. NAK00032]
MTNFPKWSDVRADVVEGAGGERGVAEARKRNQAYIDGHRLAERRKAIGLSQKEVADRMGVTKSRVSQIERGEVSTVEAIA
RYVQAIGGELQISAVFGDDMYVLRGTDTHAA

Download         Length: 336 bp

>AT160637 NZ_CP070598:4074856-4075230 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References