Detailed information of TA system TA160624

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3297433..3297658
• Replicon: chromosome
• Accession: NZ_CP054913
• Organism: Shigella flexneri strain FDAARGOS_691

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FOB99_RS16630
• Protein ID: WP_000935258.1
• Coordinates: 3297433..3297645 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3297600..3297658 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB99_RS16575	3292745..3293095	-	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-
FOB99_RS16580	3293092..3293766	-	675	WP_004967157.1	IS66 family insertion sequence hypothetical protein	-
FOB99_RS16585	3293865..3294080	-	216	WP_000839572.1	class II holin family protein	-
FOB99_RS16610	3294881..3295564	-	684	WP_005049343.1	antiterminator	-
FOB99_RS16615	3295561..3295926	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
FOB99_RS16620	3295927..3296985	-	1059	WP_001265248.1	DUF968 domain-containing protein	-
FOB99_RS16625	3296987..3297265	-	279	WP_011069426.1	hypothetical protein	-
FOB99_RS16630	3297433..3297645	-	213	WP_000935258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3297600..3297658	+	59	-	-	Antitoxin
FOB99_RS16635	3298240..3298656	-	417	WP_005069274.1	hypothetical protein	-
FOB99_RS16640	3298683..3298823	+	141	Protein_3229	DUF4224 domain-containing protein	-
FOB99_RS16645	3298823..3299863	+	1041	WP_005096324.1	tyrosine-type recombinase/integrase	-
FOB99_RS16655	3300074..3300871	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
FOB99_RS16665	3301209..3302471	+	1263	Protein_3232	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	3265585..3305779	40194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7856.42 Da
• Isoelectric Point: 8.7469

>T160624 WP_000935258.1 NZ_CP054913:c3297645-3297433 [Shigella flexneri]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T160624 NZ_CP070593:c4324601-4324074 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT160624 NZ_CP054913:3297600-3297658 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin