Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1721661..1722279 Replicon chromosome
Accession NZ_CP054913
Organism Shigella flexneri strain FDAARGOS_691

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FOB99_RS08335 Protein ID WP_001291435.1
Coordinates 1721661..1721879 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag FOB99_RS08340 Protein ID WP_000344797.1
Coordinates 1721905..1722279 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB99_RS08305 1717553..1717864 - 312 WP_000409911.1 MGMT family protein -
FOB99_RS08315 1718243..1718596 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FOB99_RS08320 1718638..1720188 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FOB99_RS08325 1720352..1720822 - 471 WP_000136192.1 YlaC family protein -
FOB99_RS08330 1720938..1721489 - 552 Protein_1609 maltose O-acetyltransferase -
FOB99_RS08335 1721661..1721879 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FOB99_RS08340 1721905..1722279 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
FOB99_RS08345 1722825..1725974 - 3150 WP_001132484.1 multidrug efflux RND transporter permease subunit -
FOB99_RS08350 1725997..1727190 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1716195..1717523 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T160611 WP_001291435.1 NZ_CP054913:c1721879-1721661 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T160611 NZ_CP070593:838544-839464 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT160611 WP_000344797.1 NZ_CP054913:c1722279-1721905 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT160611 NZ_CP070593:837775-838557 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References