160610

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1684479..1685323 Replicon chromosome
Accession NZ_CP054913
Organism Shigella flexneri strain FDAARGOS_691

Toxin (Protein)


Gene name itaT Uniprot ID A0A4P7TPN5
Locus tag FOB99_RS08140 Protein ID WP_005060796.1
Coordinates 1684479..1684877 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag FOB99_RS08145 Protein ID WP_005053053.1
Coordinates 1685012..1685323 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB99_RS08125 1679647..1681377 - 1731 WP_000645013.1 hypothetical protein -
FOB99_RS08130 1681430..1683583 - 2154 WP_005060811.1 SEL1-like repeat protein -
FOB99_RS08135 1683734..1684431 + 698 Protein_1571 IS1 family transposase -
FOB99_RS08140 1684479..1684877 - 399 WP_005060796.1 GNAT family N-acetyltransferase Toxin
FOB99_RS08145 1685012..1685323 - 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
FOB99_RS08150 1685507..1686397 - 891 WP_000971346.1 heme o synthase -
FOB99_RS08155 1686409..1686738 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
FOB99_RS08160 1686738..1687352 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
FOB99_RS08165 1687342..1689333 - 1992 Protein_1577 cytochrome o ubiquinol oxidase subunit I -
FOB99_RS08170 1689355..1689852 - 498 Protein_1578 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1683928..1684431 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(52-112)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14335.69 Da        Isoelectric Point: 9.1674

>T160610 WP_005060796.1 NZ_CP054913:c1684877-1684479 [Shigella flexneri]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 399 bp

>T160610 NZ_CP070593:571575-571859 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT160610 WP_005053053.1 NZ_CP054913:c1685323-1685012 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT160610 NZ_CP070593:571339-571572 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPN5

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References