Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1602260..1602954 Replicon chromosome
Accession NZ_CP054913
Organism Shigella flexneri strain FDAARGOS_691

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag FOB99_RS07735 Protein ID WP_001263491.1
Coordinates 1602556..1602954 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag FOB99_RS07730 Protein ID WP_000554759.1
Coordinates 1602260..1602553 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB99_RS07710 1597899..1598396 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
FOB99_RS07715 1598613..1600352 - 1740 WP_005082921.1 flagellar biosynthesis protein FlhA -
FOB99_RS07720 1600297..1601082 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
FOB99_RS07725 1601153..1602208 + 1056 WP_001226172.1 DNA polymerase IV -
FOB99_RS07730 1602260..1602553 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FOB99_RS07735 1602556..1602954 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FOB99_RS07740 1602964..1603416 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
FOB99_RS07745 1603662..1604705 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
FOB99_RS07750 1604821..1605381 + 561 Protein_1495 peptide chain release factor H -
FOB99_RS07755 1605438..1606895 - 1458 WP_128622706.1 cytosol nonspecific dipeptidase -
FOB99_RS07760 1607157..1607615 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrA / gtrB / gtrII 1596078..1640505 44427


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T160609 WP_001263491.1 NZ_CP054913:1602556-1602954 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T160609 NZ_CP070593:461296-461583 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT160609 WP_000554759.1 NZ_CP054913:1602260-1602553 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT160609 NZ_CP070593:461570-461872 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References