Detailed information of TA system TA160586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1577776..1578431
• Replicon: chromosome
• Accession: NZ_CP054880
• Organism: Pseudomonas sp. 43A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H8GGM8
• Locus tag: HUW52_RS07035
• Protein ID: WP_016985613.1
• Coordinates: 1577776..1578120 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUW52_RS07040
• Protein ID: WP_016985614.1
• Coordinates: 1578117..1578431 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW52_RS07010	1572825..1573766	+	942	WP_007916947.1	ubiquinol oxidase subunit II	-
HUW52_RS07015	1573770..1575800	+	2031	WP_007916945.1	cytochrome o ubiquinol oxidase subunit I	-
HUW52_RS07020	1575804..1576427	+	624	WP_016985612.1	cytochrome o ubiquinol oxidase subunit III	-
HUW52_RS07025	1576427..1576762	+	336	WP_007916940.1	cytochrome o ubiquinol oxidase subunit IV	-
HUW52_RS07030	1576774..1577661	+	888	WP_007916939.1	heme o synthase	-
HUW52_RS07035	1577776..1578120	+	345	WP_016985613.1	hypothetical protein	Toxin
HUW52_RS07040	1578117..1578431	+	315	WP_016985614.1	helix-turn-helix domain-containing protein	Antitoxin
HUW52_RS07045	1578632..1579123	+	492	WP_016985615.1	RidA family protein	-
HUW52_RS07050	1579412..1580113	-	702	WP_016985616.1	hypothetical protein	-
HUW52_RS07055	1580232..1581749	-	1518	WP_175553255.1	HAMP domain-containing histidine kinase	-
HUW52_RS07060	1581746..1582456	-	711	WP_175553256.1	response regulator transcription factor	-
HUW52_RS07065	1582608..1583126	+	519	WP_175553257.1	peptide-methionine (R)-S-oxide reductase MsrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13327.20 Da
• Isoelectric Point: 9.6212

>T160586 WP_016985613.1 NZ_CP054880:1577776-1578120 [Pseudomonas sp. 43A]
MRTVFFETTIFTTSVGQYLTDDEYRELQAYMQGNPLAGDVMPRTGGFRKLRWADARRGKGRRGGLRVIYYWLMNDGQFWM
FAIYDKDELDNLTAEQEKALKKAIDKELKTRGTP

Nucleotide

• Length: 345 bp

>T160586 NZ_CP070579:c4932006-4931659 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11841.80 Da
• Isoelectric Point: 10.3048

>AT160586 WP_016985614.1 NZ_CP054880:1578117-1578431 [Pseudomonas sp. 43A]
MKKRDLFAELMQGVDEMAAQREGKITLRNTVLEDKPVPEVGAQEIVALRERLHMSQAVFAKRIRASPSTLRNWEQEKSKP
NAQAALLIRLVEKFPDMVDRLSAV

Nucleotide

• Length: 315 bp

>AT160586 NZ_CP070579:c4931659-4931360 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A7H8GGM8