Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 98725..99379 Replicon chromosome
Accession NZ_CP054873
Organism Pseudomonas protegens strain PGNR2

Toxin (Protein)


Gene name higB Uniprot ID A0A2C9EQ14
Locus tag HUT21_RS00445 Protein ID WP_015636300.1
Coordinates 99029..99379 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q4K9S2
Locus tag HUT21_RS00440 Protein ID WP_011062198.1
Coordinates 98725..99042 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUT21_RS00420 (HUT21_00420) 94028..94780 - 753 WP_015636303.1 UDP-2,3-diacylglucosamine diphosphatase -
HUT21_RS00425 (HUT21_00425) 94777..95280 - 504 WP_011062201.1 peptidylprolyl isomerase -
HUT21_RS00430 (HUT21_00430) 95551..97251 + 1701 WP_015636302.1 glutamine--tRNA ligase/YqeY domain fusion protein -
HUT21_RS00435 (HUT21_00435) 97270..98652 + 1383 WP_015636301.1 cysteine--tRNA ligase -
HUT21_RS00440 (HUT21_00440) 98725..99042 - 318 WP_011062198.1 transcriptional regulator Antitoxin
HUT21_RS00445 (HUT21_00445) 99029..99379 - 351 WP_015636300.1 hypothetical protein Toxin
HUT21_RS00450 (HUT21_00450) 99617..101080 - 1464 WP_015636299.1 NADH-quinone oxidoreductase subunit NuoN -
HUT21_RS00455 (HUT21_00455) 101088..102620 - 1533 WP_015636298.1 NADH-quinone oxidoreductase subunit M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(48-101)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 14008.14 Da        Isoelectric Point: 8.9805

>T160558 WP_015636300.1 NZ_CP054873:c99379-99029 [Pseudomonas protegens]
MNALFIELPAFARYRKGFLDDELFCELQIELLRHPHAGVLIQGTGGLRKMRFIDERRNKGRRGGLRVIYYWWSEGAQFWL
FTLFDKEQMDDLLPQQRQQLKQLLEREIEARRHDQT

Download         Length: 351 bp

>T160558 NZ_CP070569:2800103-2800372 [Proteus mirabilis]
ATGATGGAGATATTCTGGACCATACTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTATATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12064.95 Da        Isoelectric Point: 10.4439

>AT160558 WP_011062198.1 NZ_CP054873:c99042-98725 [Pseudomonas protegens]
MTKRDIFSELVEGIDALTQERQGKITLRSHKVKLEKLPPITAAEVMNIRQQLNLSRSVFAMYLRTNTRTLENWEQGRAIP
NAQATTLIRLVERYPDTVERLASLA

Download         Length: 318 bp

>AT160558 NZ_CP070569:2799837-2800106 [Proteus mirabilis]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACTTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGATATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATAAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2C9EQ14

Antitoxin

Source ID Structure
AlphaFold DB A0A2C9EPZ9

References