Detailed information of TA system TA160543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4230301..4230955
• Replicon: chromosome
• Accession: NZ_CP054867
• Organism: Pseudomonas chlororaphis strain PCLRT02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A554PC23
• Locus tag: HUT27_RS18625
• Protein ID: WP_041986396.1
• Coordinates: 4230301..4230651 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUT27_RS18630
• Protein ID: WP_009049793.1
• Coordinates: 4230641..4230955 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT27_RS18615 (HUT27_18615)	4227051..4228583	+	1533	WP_009049790.1	NADH-quinone oxidoreductase subunit M	-
HUT27_RS18620 (HUT27_18620)	4228591..4230054	+	1464	WP_007929613.1	NADH-quinone oxidoreductase subunit NuoN	-
HUT27_RS18625 (HUT27_18625)	4230301..4230651	+	351	WP_041986396.1	toxin	Toxin
HUT27_RS18630 (HUT27_18630)	4230641..4230955	+	315	WP_009049793.1	transcriptional regulator	Antitoxin
HUT27_RS18635 (HUT27_18635)	4231122..4232864	-	1743	WP_023964376.1	ABC transporter substrate-binding protein	-
HUT27_RS18640 (HUT27_18640)	4233028..4233300	-	273	WP_009049795.1	DUF2160 domain-containing protein	-
HUT27_RS18645 (HUT27_18645)	4233311..4234111	-	801	WP_124355220.1	carbohydrate ABC transporter permease	-
HUT27_RS18650 (HUT27_18650)	4234123..4234989	-	867	WP_009049796.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13861.80 Da
• Isoelectric Point: 8.5032

>T160543 WP_041986396.1 NZ_CP054867:4230301-4230651 [Pseudomonas chlororaphis]
MDALFIELPPFQRHRQDYLDDELFRSLQLELLKAPEAGDLIEGTDGLRKIRFVDERRHKGKRGGIRVIYYWWSGGAQFWL
FTLYAKNEQNDLTPHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T160543 NZ_CP070566:c985-719 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11917.85 Da
• Isoelectric Point: 10.6052

>AT160543 WP_009049793.1 NZ_CP054867:4230641-4230955 [Pseudomonas chlororaphis]
MKRDIFSELVEGFDALAKERQGKLTLRTYKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT160543 NZ_CP070566:c1193-969 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A554PC23