160522

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5881564..5882159 Replicon chromosome
Accession NZ_CP054845
Organism Pseudomonas aeruginosa strain SE5429

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag HUS13_RS27775 Protein ID WP_003113526.1
Coordinates 5881881..5882159 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUS13_RS27770 Protein ID WP_003133769.1
Coordinates 5881564..5881869 (-) Length 102 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS13_RS27735 5876715..5877563 + 849 WP_003095007.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
HUS13_RS27745 5877730..5878671 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
HUS13_RS27750 5878788..5879402 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
HUS13_RS27755 5879444..5880028 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
HUS13_RS27760 5880069..5881169 + 1101 WP_003095017.1 redox-regulated ATPase YchF -
HUS13_RS27770 5881564..5881869 - 306 WP_003133769.1 HigA family addiction module antidote protein Antitoxin
HUS13_RS27775 5881881..5882159 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUS13_RS27780 5882488..5884716 + 2229 WP_023104079.1 TonB-dependent receptor -
HUS13_RS27785 5884787..5885434 - 648 WP_003095021.1 carbonate dehydratase -
HUS13_RS27790 5885496..5886734 - 1239 WP_003095023.1 C69 family dipeptidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-92)

Antitoxin

(6-82)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T160522 WP_003113526.1 NZ_CP054845:c5882159-5881881 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T160522 NZ_CP070549:4110240-4110524 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGCTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11214.73 Da        Isoelectric Point: 4.6081

>AT160522 WP_003133769.1 NZ_CP054845:c5881869-5881564 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Download         Length: 306 bp

>AT160522 NZ_CP070549:4110008-4110250 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTTGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V6ALY3


Antitoxin

Source ID Structure

References