Detailed information of TA system TA160516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5891923..5892518
• Replicon: chromosome
• Accession: NZ_CP054844
• Organism: Pseudomonas aeruginosa strain SE5357

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HUS11_RS28100
• Protein ID: WP_003113526.1
• Coordinates: 5892240..5892518 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUS11_RS28095
• Protein ID: WP_003113527.1
• Coordinates: 5891923..5892228 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS11_RS28075	5887999..5888256	+	258	WP_010793759.1	ATP-binding protein	-
HUS11_RS28080	5888632..5889495	-	864	WP_010793758.1	integrase domain-containing protein	-
HUS11_RS28085	5890097..5891239	-	1143	WP_010793757.1	hypothetical protein	-
HUS11_RS28095	5891923..5892228	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HUS11_RS28100	5892240..5892518	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUS11_RS28105	5892847..5895075	+	2229	WP_003099265.1	TonB-dependent receptor	-
HUS11_RS28110	5895145..5895792	-	648	WP_003095021.1	carbonate dehydratase	-
HUS11_RS28115	5895854..5897092	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T160516 WP_003113526.1 NZ_CP054844:c5892518-5892240 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160516 NZ_CP070549:2666591-2666968 [Citrobacter freundii]
GTGCAAACCCAACCACTATCCTCCACGCATGAGACCTCACCACGTCCGTCCCCCGTGGATATCTGGCAGCACCTCCTGAA
CCATCTGCTGGATCGTCACTACGGTCTGACGCTAAACGACACGCCATTTGGCAACGATGGCGTGATCCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAACTTTATCGTGGAAAAGTACGACCTGGTCCGTACTGACAGACGCGGCTTT
AGCGCAGACACACAGTCACCGCTTATCGGCAGTATTGATATCCTCCGAGCCCGGAAGGCGACCGGGCTGATGACCCGTCA
TGGCTACAGACCTGTGACAGATCTCATTACCGGCAAATACAAAAAGGAACAACAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT160516 WP_003113527.1 NZ_CP054844:c5892228-5891923 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160516 NZ_CP070549:2666213-2666539 [Citrobacter freundii]
ATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTCGGCGACCGCGCGAGTATCGCCGGCACGTTCAGCGACGCGGATTTACGCCATCTTGACCAGGCGTTTCCGG
TACAGCTCAAACAGCTTGAGCTGATGCTGGTTTCCGGTGAACTCAATCCCCGCCATCAGCACTGCGTCACATTATACTCA
AAAGGGCTGACCTGCGAGGCCGACAGCCTCGGCTCGCATGGATACGTGTACATCGCGATTTACCCGACACCTGCAGCAAC
CGCGTAA

Structures

Toxin

• AlphaFold DB: V6ALY3