Detailed information of TA system TA160484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5522353..5522948
• Replicon: chromosome
• Accession: NZ_CP054794
• Organism: Pseudomonas aeruginosa strain A0002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HUK78_RS25860
• Protein ID: WP_003113526.1
• Coordinates: 5522670..5522948 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK78_RS25855
• Protein ID: WP_003099268.1
• Coordinates: 5522353..5522658 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK78_RS25820 (HUK78_25815)	5517502..5518350	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HUK78_RS25830 (HUK78_25825)	5518517..5519458	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HUK78_RS25835 (HUK78_25830)	5519575..5520189	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HUK78_RS25840 (HUK78_25835)	5520231..5520815	+	585	WP_128698405.1	aminoacyl-tRNA hydrolase	-
HUK78_RS25845 (HUK78_25840)	5520856..5521956	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HUK78_RS25855 (HUK78_25850)	5522353..5522658	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
HUK78_RS25860 (HUK78_25855)	5522670..5522948	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK78_RS25865	5523001..5523129	-	129	Protein_5119	integrase	-
HUK78_RS25870 (HUK78_25860)	5523277..5525505	+	2229	WP_003099265.1	TonB-dependent receptor	-
HUK78_RS25875 (HUK78_25865)	5525575..5526222	-	648	WP_003095021.1	carbonate dehydratase	-
HUK78_RS25880 (HUK78_25870)	5526284..5527522	-	1239	WP_009316205.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T160484 WP_003113526.1 NZ_CP054794:c5522948-5522670 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160484 NZ_CP070528:1846552-1846884 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT160484 WP_003099268.1 NZ_CP054794:c5522658-5522353 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160484 NZ_CP070528:1846294-1846551 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: V6ALY3