Detailed information of TA system TA160478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5420428..5421023
• Replicon: chromosome
• Accession: NZ_CP054793
• Organism: Pseudomonas aeruginosa strain SE5452

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HUK77_RS25245
• Protein ID: WP_003117425.1
• Coordinates: 5420745..5421023 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK77_RS25240
• Protein ID: WP_003113527.1
• Coordinates: 5420428..5420733 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK77_RS25205 (HUK77_25200)	5415568..5416416	+	849	WP_003117426.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HUK77_RS25215 (HUK77_25210)	5416583..5417524	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HUK77_RS25220 (HUK77_25215)	5417641..5418255	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HUK77_RS25225 (HUK77_25220)	5418297..5418881	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HUK77_RS25230 (HUK77_25225)	5418922..5420022	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HUK77_RS25240 (HUK77_25235)	5420428..5420733	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
HUK77_RS25245 (HUK77_25240)	5420745..5421023	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK77_RS25250	5421076..5421204	-	129	Protein_4995	integrase	-
HUK77_RS25255 (HUK77_25245)	5421352..5423580	+	2229	WP_003113525.1	TonB-dependent receptor	-
HUK77_RS25260 (HUK77_25250)	5423650..5424297	-	648	WP_003095021.1	carbonate dehydratase	-
HUK77_RS25265 (HUK77_25255)	5424359..5425597	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T160478 WP_003117425.1 NZ_CP054793:c5421023-5420745 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160478 NZ_CP070524:c2419036-2418683 [Morganella morganii]
GTGTGGAAGATAGTCACAACAGATAATTTTACAGCGTGGTTTGCTGAGCAGGATGACGGAGATCGCGCAGCAATACTGGC
AGCGATGCTGGTATTGGAGAAGCGGGGACCCGGCTTACCAAGGCCGTATGCGGATACTGTAAAAGGTTCCCGGTATACGA
ATATGAAAGAGCTTCGTATACAGAGCAGGGGACAACCGATACGGGCATTTTTTGCATTCGACCCTGAGAGAAGAGGCGTC
TTGCTGTGTGCGGGGTATAAAACCGGCAAAGAAAAACGTTTTTATATGCAGATGATCCCGCAGGCTGACCGTGAGTATAC
AGAATGGCTGAACAGGCCGGAAAACAGGAGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT160478 WP_003113527.1 NZ_CP054793:c5420733-5420428 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160478 NZ_CP070524:c2418680-2418378 [Morganella morganii]
ATGGGAAAGTCACTCAGACAACTGCTGGCTGAGGAAAAGCCGGAAGTCGCGGCTGCTGCACGGGTTCAGGCGGATGAGAT
CCTGCTGAATATCCATCTGGCGGAGCTGCGGGAGAAAGTGAAGAAAACACAGGTGGAAATGGCGCAGGCACTGGGAATAA
AACAGCCGACAGTCGCAGGGATGGAAAAACCGGGGCGTGATGTCAAATTATCCACGCTGAGACGCTATGTGGATGCCATC
GGCGGAAAACTGAATATTGATGTTGAACTGCCGGACGGCACGCATTATATGTTCCGGGTCTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5