Detailed information of TA system TA160471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5580380..5580975
• Replicon: chromosome
• Accession: NZ_CP054792
• Organism: Pseudomonas aeruginosa strain SE5431

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HUK75_RS26350
• Protein ID: WP_003113526.1
• Coordinates: 5580697..5580975 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK75_RS26345
• Protein ID: WP_003133769.1
• Coordinates: 5580380..5580685 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK75_RS26315 (HUK75_26265)	5576405..5576716	+	312	WP_033988980.1	hypothetical protein	-
HUK75_RS26320 (HUK75_26270)	5576719..5576940	-	222	WP_033988977.1	hypothetical protein	-
HUK75_RS26325 (HUK75_26275)	5576940..5577578	-	639	WP_033988974.1	DNA-binding protein	-
HUK75_RS26330 (HUK75_26280)	5578553..5579467	-	915	WP_228846195.1	hypothetical protein	-
HUK75_RS26335 (HUK75_26290)	5579506..5579718	-	213	WP_033988972.1	AlpA family phage regulatory protein	-
HUK75_RS26345 (HUK75_26300)	5580380..5580685	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
HUK75_RS26350 (HUK75_26305)	5580697..5580975	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK75_RS26355	5581028..5581156	-	129	Protein_5219	integrase	-
HUK75_RS26360 (HUK75_26310)	5581304..5583532	+	2229	WP_033988971.1	TonB-dependent receptor	-
HUK75_RS26365 (HUK75_26315)	5583602..5584249	-	648	WP_003095021.1	carbonate dehydratase	-
HUK75_RS26370 (HUK75_26320)	5584311..5585549	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T160471 WP_003113526.1 NZ_CP054792:c5580975-5580697 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160471 NZ_CP070520:c2641791-2641381 [Klebsiella aerogenes]
GTGGTCCTGTGGCAATCTGATTTACGTATTTCCTGGCGTTCGCAGTGGTTCTCGCTGCTGCTGCACGGGGTGGTGGCGGC
GATAGTGCTGCTCATGCCCTGGCCGTTAAGCTACACCCCGATTTGGCTCCTGTTGCTATCGCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCACGCCTGCCAGGGAGAAATTAAGCTGTTGATCGATTCCCGTTTACGGTGGCAAAAAACG
GAATGGGATATCGTCGGGACGCCATGGGTTATCAGCAGCGGGATGCTGCTGCGCCTGAAGCATGCGGAAAGCGGCCGTAG
CCAGCATTTGTGGGTGGCGGCGGATAGCATGGATGCGGGGGAATGGCGGGATTTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCACGACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT160471 WP_003133769.1 NZ_CP054792:c5580685-5580380 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160471 NZ_CP070520:c2642038-2641772 [Klebsiella aerogenes]
ATGGACATTAACAACAAAGCTCGCATCCACTGGGCATGCCGCCGCGGTATGCGCGAACTCGATATCTCCATCATGCCGTT
TTTCGAGTATGAGTACGACACGCTCAGCGATGACGACAAGCGCCTGTTCATCCGTTTGCTGGAGAACGATGATCCGGATC
TGTTTAACTGGCTGATGAATCACGGCAAGCCGGCAGATGCGCAACTTCAACGGATGATTAATTTAATCCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3