Detailed information of TA system TA160465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5712770..5713365
• Replicon: chromosome
• Accession: NZ_CP054791
• Organism: Pseudomonas aeruginosa strain SE5430

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HUK74_RS27070
• Protein ID: WP_003113526.1
• Coordinates: 5713087..5713365 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK74_RS27065
• Protein ID: WP_003133769.1
• Coordinates: 5712770..5713075 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK74_RS27030 (HUK74_27000)	5707921..5708769	+	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HUK74_RS27040 (HUK74_27010)	5708936..5709877	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HUK74_RS27045 (HUK74_27015)	5709994..5710608	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
HUK74_RS27050 (HUK74_27020)	5710650..5711234	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HUK74_RS27055 (HUK74_27025)	5711275..5712375	+	1101	WP_003095017.1	redox-regulated ATPase YchF	-
HUK74_RS27065 (HUK74_27035)	5712770..5713075	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
HUK74_RS27070 (HUK74_27040)	5713087..5713365	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK74_RS27075	5713418..5713546	-	129	Protein_5361	integrase	-
HUK74_RS27080 (HUK74_27045)	5713694..5715922	+	2229	WP_023104079.1	TonB-dependent receptor	-
HUK74_RS27085 (HUK74_27050)	5715993..5716640	-	648	WP_003095021.1	carbonate dehydratase	-
HUK74_RS27090 (HUK74_27055)	5716702..5717940	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T160465 WP_003113526.1 NZ_CP054791:c5713365-5713087 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160465 NZ_CP070509:c33578-33297 [Serratia ureilytica]
ATGACTTATAAACTCAAGTTTGAGAAGCGGGCGCTGAAAGAGTGGGAAAAGCTGGGGCATCCGATCAGGGAGCAGCTGAA
AAAAAAGCTGGCCGAGCGATTGGAGAATCCACATGTCCCAGCGTCCCGACTCAGTGGCCGCCAGAACCGCTACAAAATCA
AACTACGTTCGTCAGGCTATCGACTGGTTTACCAAGTCATCGACAACGAAATCGTCCTGCTGGTGATCGCTGTTGGCAAA
CGGGCAGGGGATGAAGTCTATTTAACCGCCGACAAACGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT160465 WP_003133769.1 NZ_CP054791:c5713075-5712770 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160465 NZ_CP070509:c33819-33568 [Serratia ureilytica]
ATGGCTTACCAGATCTTAACCACTACTGCGGCGTCTATCACCGACCTGAAAAAAAATCCGATGGGAACCGTTGCCGACGG
AGACGGTGAAGCGATCGCGATCCTCAATCGCAACGAACCGGCGTTCTACTGCGTTCCCCCTGAGCTTTTTGCATACTATC
GCGAACTGGCCGAAGATGCGGAGCTCAATCAAATCGCTGATGAGCGCATGAAAGATGCCGAGTACGTGAGCGTTTCTCTC
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: V6ALY3