Detailed information of TA system TA160459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5864439..5865034
• Replicon: chromosome
• Accession: NZ_CP054790
• Organism: Pseudomonas aeruginosa strain SE5418

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HUK71_RS27930
• Protein ID: WP_003113526.1
• Coordinates: 5864756..5865034 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK71_RS27925
• Protein ID: WP_003113527.1
• Coordinates: 5864439..5864744 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK71_RS27905	5860515..5860772	+	258	WP_010793759.1	ATP-binding protein	-
HUK71_RS27910 (HUK71_27900)	5861148..5862011	-	864	WP_010793758.1	integrase domain-containing protein	-
HUK71_RS27915 (HUK71_27905)	5862613..5863755	-	1143	WP_010793757.1	STY4528 family pathogenicity island replication protein	-
HUK71_RS27925 (HUK71_27915)	5864439..5864744	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
HUK71_RS27930 (HUK71_27920)	5864756..5865034	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK71_RS27935	5865087..5865215	-	129	Protein_5538	integrase	-
HUK71_RS27940 (HUK71_27925)	5865363..5867591	+	2229	WP_003099265.1	TonB-dependent receptor	-
HUK71_RS27945 (HUK71_27930)	5867661..5868308	-	648	WP_003095021.1	carbonate dehydratase	-
HUK71_RS27950 (HUK71_27935)	5868370..5869608	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T160459 WP_003113526.1 NZ_CP054790:c5865034-5864756 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160459 NZ_CP070508:c1120154-1119951 [Serratia ureilytica]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAAAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCCGCCGATCATCGTTTGGCGGAGCTGACCATGAACAAGC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT160459 WP_003113527.1 NZ_CP054790:c5864744-5864439 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160459 NZ_CP070508:c1120573-1120205 [Serratia ureilytica]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAACTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
CACCCTGGGAGACAGCCACCACGGGTGGGTCAACGATCCGACATCCTCCGTCAACCTTCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCCGATTTGTCGGAGTTAGTCGAGGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: V6ALY3