Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5704377..5704972 Replicon chromosome
Accession NZ_CP054789
Organism Pseudomonas aeruginosa strain SE5381

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag HUK70_RS27080 Protein ID WP_003113526.1
Coordinates 5704694..5704972 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUK70_RS27075 Protein ID WP_003113527.1
Coordinates 5704377..5704682 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUK70_RS27055 5700453..5700710 + 258 WP_010793759.1 ATP-binding protein -
HUK70_RS27060 (HUK70_26975) 5701086..5701949 - 864 WP_010793758.1 integrase domain-containing protein -
HUK70_RS27065 (HUK70_26980) 5702551..5703693 - 1143 WP_010793757.1 STY4528 family pathogenicity island replication protein -
HUK70_RS27075 (HUK70_26990) 5704377..5704682 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
HUK70_RS27080 (HUK70_26995) 5704694..5704972 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUK70_RS27085 5705025..5705153 - 129 Protein_5369 integrase -
HUK70_RS27090 (HUK70_27000) 5705301..5707529 + 2229 WP_003099265.1 TonB-dependent receptor -
HUK70_RS27095 (HUK70_27005) 5707599..5708246 - 648 WP_003095021.1 carbonate dehydratase -
HUK70_RS27100 (HUK70_27010) 5708308..5709546 - 1239 WP_010793756.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T160451 WP_003113526.1 NZ_CP054789:c5704972-5704694 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T160451 NZ_CP070506:5941458-5942336 [Pseudomonas sp. SDM007]
ATGTTGCCAGGACTCACTCAAGCCGCGCCGACGGCCAATCTGCAAGCCCAACTGCTGCGCGATTTCCCCCAGCAACGGGT
CGCCTCAGCAGGTGGCCAACAGGCCCTGCGCGAATGCGGCACGGGGCCTGCGGTGGTGTTGCTGCACGGCATCGGTTCCG
GCGCGGCGTCCTGGCTGCAGGTGGCACAGCATCTGGCGGCCCAGGCGCGGGTCATCGCCTGGGACGCACCCGGCTATGGC
GACTCCAGCCCCCTGGAGTCGCAAGTACCCAAGGCCGAGCAATACGCCGCGCGCCTGGTGCAGATGCTCGACGCGCTGCA
GGTGGATGAGTGCGTGCTGGTCGGCCATTCCCTGGGCGCGCTGACCGCCCTGGCATTGGCCCGCAGTGCCCAGGCCAAAC
GCGTGAGCCGCCTGGTGCTGATCAGCCCCGCCCGCGGCTATGGCGCGCCCGAACGTAGCGCCCAGCGCCAGCAAGTACGC
CAGCAACGCCTGGACAACCTGCAACGCCTGGGCATCGCCGGGCTGGCCGCACAACGCAGCGCGCACATGCTGTCGCCCCG
TGCCCAGCCCGAAGCATTGGCCTGGGTCCACTGGAACATGGCGCGCCTGAACCCGCTGGGTTATCGCCAGGCCATCGAAT
TGCTGTGCGGCGACGACCTGCTGCGCCACGGCCAACCGGCGATGCCCTGCGACGTGCACTGCGGCGAAGCCGACGCCATC
ACCACCCCCGACACCTGCCTGGGCGTGGCCAAGGCCCTGGGTGCCAGCTTCAACCTGATCGCCGACGCAGGCCATGCCAG
CCCCATCGAACAACCGCTGGTGGTTGCCGGTCGCCTGGCCTACGCCCTCAAACAATCCCTGACAGGTAGAACCTTATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT160451 WP_003113527.1 NZ_CP054789:c5704682-5704377 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT160451 NZ_CP070506:5942333-5943166 [Pseudomonas sp. SDM007]
ATGAATGACTCAGATCTGCTGAAGGATGCCCAGGACAAATACATCGTGCCGGGCCTGGAGCGCGGCTTGCTGCTGCTCTG
CGAGTTCAGCCGGCGCAACCGCACCCTGACGGCACCAGAGCTGGCACGGCGCCTGGCGCTGCCGCGCTCGACCATCTTCC
GCCTGCTGACCACCCTGGAAACCATGGGCTTCGTCACCCGCAGCGGCAACGAATACCGCCTGGGCATGTCGGTGCTGCGC
CTGGGCTTCGAGTACCTCGCGTCGCTGGAGCTGACCGAGCTGGGGCAGCCACTGTTGGCGCGGTTGTGCGACCGGGTGAA
CTACCCCAGCAACCTGGTAGTGCGCGACGGACGTTCCATCGTGTACGTGGCCAAGGTCTCGCCGCCTTCGGTGTTCTCCA
ACGCGGTCAACGTCGGCACCCGGTTGCCGGCCCATGCCACGGTGCTGGGGCGCATCCTTCTCGAAGACCTGTCCCTGGCC
GAACTGCGCGAGCTGTACCCCGAGGAGCATCTGGAACAGCACTCTCACTGCACGCCCAAAACCGTGATGGAGCTGTTCGA
CTTGCTCCAGGCCGACCGCCAGCGCGGCTTTGTCAGCGGCGAAGGGTTCTTTGAATCGGCGATTTCCACAGTCGCCGCGC
CCGTACGCGACCAGAGCGGCGACATCGTTGCGGCCCTTGGCGTGACCATCCCCACCACGCAAATCGGTCATGTGAATTTT
GAAGAGCTGTTGACCCAGGTCCGGCGCAGCGCCGACGAGTTGTCGCGCCTGCTCAACTACAACGGCGGCGCCCACAGCGG
CCAGCCCCGCGTCACTGCCTTGATGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References