Detailed information of TA system TA160444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5684609..5685204
• Replicon: chromosome
• Accession: NZ_CP054788
• Organism: Pseudomonas aeruginosa strain YTSY4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HUK69_RS27065
• Protein ID: WP_003113526.1
• Coordinates: 5684926..5685204 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK69_RS27060
• Protein ID: WP_003099268.1
• Coordinates: 5684609..5684914 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK69_RS27025 (HUK69_26990)	5679750..5680598	+	849	WP_235195670.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HUK69_RS27035 (HUK69_27000)	5680765..5681706	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HUK69_RS27040 (HUK69_27005)	5681823..5682437	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
HUK69_RS27045 (HUK69_27010)	5682479..5683063	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HUK69_RS27050 (HUK69_27015)	5683104..5684204	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HUK69_RS27060 (HUK69_27025)	5684609..5684914	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
HUK69_RS27065 (HUK69_27030)	5684926..5685204	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK69_RS27070	5685257..5685385	-	129	Protein_5362	integrase	-
HUK69_RS27075 (HUK69_27035)	5685533..5687761	+	2229	WP_042180315.1	TonB-dependent receptor	-
HUK69_RS27080 (HUK69_27040)	5687831..5688478	-	648	WP_003095021.1	carbonate dehydratase	-
HUK69_RS27085 (HUK69_27045)	5688540..5689778	-	1239	WP_017001387.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T160444 WP_003113526.1 NZ_CP054788:c5685204-5684926 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160444 NZ_CP070504:55436-55735 [Pseudomonas atacamensis]
ATGGAAAAAAGAACCCCACACTGCAAGCTGTCCGCCGTCAAAGCCTTGATTGGTGCGGGCAAAGTGAAATCGACCACAAC
CGCGCTCGCTGGCGCTGCTGCGTTGGGTTTCGACTTTGAAGGGATGGTCGGCATCGTAATGCTGCTCAAGCCTGCCGACT
TCTACAAGAGCATGACCACCTACGCCGACCACACCACTTGGCAAGACGTCTACCGTCCGAAGCTGGGCGATGGTCGCCGG
GTCTATCTGAAACTCACTGTGACCGATGACGCGTTGATCGTCTCGTTCAAGGAGCAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT160444 WP_003099268.1 NZ_CP054788:c5684914-5684609 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160444 NZ_CP070504:55737-56138 [Pseudomonas atacamensis]
ATGAAATGTCCAATCTGTGGGGGCGCGGAGCTGGTGACAGACACCCGCGACCTGCCGTACACCTACAAAAACGAATCGAC
CGTTATCCCGGCCGTGCACGGCGAGTTTTGCTCTGCGTGCGGGGAATCGATTCTGGGTGTTGCTGAGTCGAACCGTGTAA
GCGCGGCAATGCTGGCTTTCAACAAGACGGTCAACGCGGCCGTGGTAGATCCGCTGTTCATCACCAGCGTGCGCAAGAAG
CTGGCGCTCGATCAGCGCCAAGCCGCTGAAATCTTCGGCGGGGGCACCAATGCGTTCTCTCGGTATGAAACGGGGAAGGT
AAAGCCACCGATCGCGCTGGTGCTGTTGCTCAAGCTGCTCGATCGTCACCCTGAATTGCTGAATGAAGTGAGAGCGGCAT
GA

Structures

Toxin

• AlphaFold DB: V6ALY3