Detailed information of TA system TA160442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4936929..4937610
• Replicon: chromosome
• Accession: NZ_CP054788
• Organism: Pseudomonas aeruginosa strain YTSY4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HUK69_RS23415
• Protein ID: WP_031667792.1
• Coordinates: 4937245..4937610 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK69_RS23410
• Protein ID: WP_003145733.1
• Coordinates: 4936929..4937252 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK69_RS23385 (HUK69_23350)	4932842..4933480	+	639	WP_126861196.1	hypothetical protein	-
HUK69_RS23390 (HUK69_23355)	4933723..4934058	+	336	WP_003119438.1	TM2 domain-containing protein	-
HUK69_RS23395 (HUK69_23360)	4934232..4934750	+	519	WP_024928874.1	PAAR domain-containing protein	-
HUK69_RS23400 (HUK69_23365)	4934747..4935448	+	702	WP_235195645.1	VRR-NUC domain-containing protein	-
HUK69_RS23405 (HUK69_23370)	4935465..4936553	+	1089	WP_003145732.1	DUF3396 domain-containing protein	-
HUK69_RS23410 (HUK69_23375)	4936929..4937252	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HUK69_RS23415 (HUK69_23380)	4937245..4937610	-	366	WP_031667792.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK69_RS23420 (HUK69_23385)	4937903..4938143	-	241	Protein_4646	hypothetical protein	-
HUK69_RS23425 (HUK69_23390)	4938350..4938622	+	273	WP_003116491.1	hypothetical protein	-
HUK69_RS23430 (HUK69_23395)	4938653..4939078	-	426	WP_003085665.1	VOC family protein	-
HUK69_RS23435 (HUK69_23400)	4939179..4940063	+	885	WP_003085663.1	LysR substrate-binding domain-containing protein	-
HUK69_RS23440 (HUK69_23405)	4940036..4940989	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
HUK69_RS23445 (HUK69_23410)	4941210..4941644	+	435	WP_016253806.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13812.17 Da
• Isoelectric Point: 4.8298

>T160442 WP_031667792.1 NZ_CP054788:c4937610-4937245 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDERWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T160442 NZ_CP070503:c5566653-5566291 [Pseudomonas atacamensis]
ATGGTGCGCCGACAGCCCCCTCAGCGGGGTGACGTTTACTGGATTGATTTAAACCCAGTGGCGGGGCGGGAGATGATGAA
CAGACATCGCTTTGTGGTGATCACGCCCAGAGAGATCAACGCATTGGGCGTGAGCATGACGGTGCCTGTTACTAGCGGTG
GGAATTTCAGCCGGAATATGGGTTTGGTGGTCGCCATTACTGGCCACGAAACTAATGGTGTAGCGGTGTGTAATCAGGTT
CGCAGTTTTGACATAGAACAACGGGTACGAGACGGCACGGCCAAATTCATCGAGCGACTGGATGATGTGACAATGTCTGA
CATCGTCGACCGTGTAATCAGTGCAATCGACCCGCTAAGCTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT160442 WP_003145733.1 NZ_CP054788:c4937252-4936929 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT160442 NZ_CP070503:c5566922-5566653 [Pseudomonas atacamensis]
ATGTCCGTAACTTCCAAGATACGCCGCCAAGGCGGCGCTGCCGTCATTACGATTCCGCCCACGCTTTTAAAGTTGTTGCA
TCTGGAGGTTGGCGCACAGCTAGAGCTGAATGTGGTCGATGGCGAACTGATTGCTCGTCCAGCCGTAGCCGCTACGCGTA
AGCGTTATAGTTTGTCAGAGCTACTCGAAGGCGCAGAAGAATTGCAGAAACTTAACGCCCAGACTAGCGAGACCCTTGAA
GGTGATTCTGTCGGCCGGGAGCTGGGTTGA