Detailed information of TA system TA160435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5714913..5715508
• Replicon: chromosome
• Accession: NZ_CP054787
• Organism: Pseudomonas aeruginosa strain HB2011305RE

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HUK67_RS27150
• Protein ID: WP_003117425.1
• Coordinates: 5715230..5715508 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK67_RS27145
• Protein ID: WP_003099268.1
• Coordinates: 5714913..5715218 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK67_RS27110 (HUK67_27100)	5710052..5710900	+	849	WP_003162406.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HUK67_RS27120 (HUK67_27110)	5711067..5712008	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HUK67_RS27125 (HUK67_27115)	5712125..5712739	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HUK67_RS27130 (HUK67_27120)	5712781..5713365	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HUK67_RS27135 (HUK67_27125)	5713406..5714506	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HUK67_RS27145 (HUK67_27135)	5714913..5715218	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
HUK67_RS27150 (HUK67_27140)	5715230..5715508	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK67_RS27155	5715561..5715685	-	125	Protein_5375	integrase	-
HUK67_RS27160 (HUK67_27145)	5715833..5718061	+	2229	WP_003113525.1	TonB-dependent receptor	-
HUK67_RS27165 (HUK67_27150)	5718131..5718778	-	648	WP_003095021.1	carbonate dehydratase	-
HUK67_RS27170 (HUK67_27155)	5718840..5720078	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T160435 WP_003117425.1 NZ_CP054787:c5715508-5715230 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160435 NZ_CP070501:2642517-2642798 [Agrococcus sp. Marseille-Q4369]
GTGATCAGATCGTTCGGCGACAAGGAGACCGAACGGCTGTGGCGTCGTGAACGCGTGAGGTCGATTGATCCGAGGATCCA
TCGAGTCGCATTGCGCAAACTTCGTCAGGTGGGGTCCGCGGAGTCCATCGAGGACCTCCGTGTCCCACCCGGCAACCGCC
TCGAAGCTCTCAAGGGCGACCGTGCCGGGCAGCACAGCATCAGGATCAACGACCAGTGGCGGATCTGCTTCGTGTGGACC
GCCGCCGGACCAGAGGAGGTGCAGATCGTTGACGACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT160435 WP_003099268.1 NZ_CP054787:c5715218-5714913 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160435 NZ_CP070501:2642785-2643087 [Agrococcus sp. Marseille-Q4369]
TTGACGACCACTGACAAGATCGAGCCGATCCACCCGGGCGAGGTGCTCATGGAGGACTTCATCGAGGGCTTCGGCATCAC
GCAGAACAAGCTCGCCGTATCCATCGGCGTGCCGCCGCGACGGATCAACGAGATCGTGCACGGCAAGCGAGGGATCACCG
CAGACACGGCGCTGCGGCTCGCGAAGTACTTCGGCACCTCGGCCGAGTTCTGGATCAACCTGCAGAGCCATTACGAGCTT
GACCGCGCCGAGGACGCGGCAGGTGAGCAGATCGCGGCGATCGCGCCGCTGCAGGTCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5