Detailed information of TA system TA160425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3428734..3429329
• Replicon: chromosome
• Accession: NZ_CP054786
• Organism: Pseudomonas aeruginosa strain DL201330

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HUK64_RS16015
• Protein ID: WP_003117425.1
• Coordinates: 3428734..3429012 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUK64_RS16020
• Protein ID: WP_003099268.1
• Coordinates: 3429024..3429329 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK64_RS15995 (HUK64_15975)	3424164..3425402	+	1239	WP_014603324.1	C69 family dipeptidase	-
HUK64_RS16000 (HUK64_15980)	3425464..3426111	+	648	WP_003095021.1	carbonate dehydratase	-
HUK64_RS16005 (HUK64_15985)	3426181..3428409	-	2229	WP_003113525.1	TonB-dependent receptor	-
HUK64_RS16010	3428557..3428681	+	125	Protein_3164	integrase	-
HUK64_RS16015 (HUK64_15990)	3428734..3429012	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK64_RS16020 (HUK64_15995)	3429024..3429329	+	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
HUK64_RS16030 (HUK64_16005)	3429736..3430836	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HUK64_RS16035 (HUK64_16010)	3430877..3431461	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HUK64_RS16040 (HUK64_16015)	3431503..3432117	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HUK64_RS16045 (HUK64_16020)	3432234..3433175	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HUK64_RS16055 (HUK64_16030)	3433342..3434190	-	849	WP_003162406.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T160425 WP_003117425.1 NZ_CP054786:3428734-3429012 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T160425 NZ_CP070499:1808732-1809124 [Natronosporangium hydrolyticum]
GTGACGTTCTTGCTGGATGCGAACGTCGTGGTTGCCCTGTGCGCTGCCGACCATGTCCACCACGAGGTGGCCTCAGCCTG
GTTTTCCCAGACGCAGGAACCGTTCGCGACGACTCCGATCACCCAGGGCTCGGTATTACGCTTCTTGCTCAGGGAAGGTG
TGGATGCTGCTAACGCGCTTGAGGTAATGGGCAGCGTGACCGGTCACCCACGTCACGAGTTCTGGCCGGGCGATCTTCCA
TTCACCAACGAGACGCTCCGGGGTGTGGTCGGGCACCGTCAGGTGACCGATGCCTACCTTGCCAACCAGGCGCGACGGCG
AGGTGGCAAGGTTGCCACGCTCGATAAAGGATTGGTCGCGCTCCACGCAGACGTGGCTACGCTGGTACGTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT160425 WP_003099268.1 NZ_CP054786:3429024-3429329 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT160425 NZ_CP070499:1808508-1808735 [Natronosporangium hydrolyticum]
ATGCGTACAACTGTTGATCTTCCTGACGACCTGCACCAGATGGTGACAGCGGTCGCTCGGGATGAAGGCAAGAGCATGAG
TCGGGCGATCGCCGACTTGCTGCGCCGCCAATTGGCGCCGCCGGCCGGGCCGCCGATGAGCACTGACGAGCGCACTGGTC
TCCGGGTGGTCCGGCTGGGCCGCCCGATCACCAGCGAGGACGTTCGTTCGCTGGAGGACGAGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5