Detailed information of TA system TA160421

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132675..132928
• Replicon: plasmid pKP20194a-p2
• Accession: NZ_CP054782
• Organism: Klebsiella pneumoniae strain KP20194a

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUR98_RS28765
• Protein ID: WP_001312851.1
• Coordinates: 132779..132928 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	132675..132734 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR98_RS28725	128057..128401	-	345	Protein_157	IS6-like element IS26 family transposase	-
HUR98_RS28730	128453..129157	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HUR98_RS28735	129221..129382	+	162	Protein_159	DNA helicase	-
HUR98_RS28740	129402..130148	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HUR98_RS28745	130203..130763	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HUR98_RS28750	130895..131095	+	201	WP_015059022.1	hypothetical protein	-
HUR98_RS28755	131481..132080	+	600	WP_032083981.1	hypothetical protein	-
HUR98_RS28760	132244..132474	+	231	WP_001736714.1	hypothetical protein	-
-	132675..132734	-	60	NuclAT_1	-	Antitoxin
-	132675..132734	-	60	NuclAT_1	-	Antitoxin
-	132675..132734	-	60	NuclAT_1	-	Antitoxin
-	132675..132734	-	60	NuclAT_1	-	Antitoxin
HUR98_RS28765	132779..132928	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUR98_RS28770	133212..133460	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133771	133771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T160421 WP_001312851.1 NZ_CP054782:132779-132928 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T160421 NZ_CP070499:145748-146182 [Natronosporangium hydrolyticum]
GTGATCATCGTTGATGTCAATCTGCTGCTGTACGCGGTCATCACCGGTTTCGCCGAACACCAGCGCGCCAGGGCGTGGTT
CGAGGCGACCCTGAACAGCCCGACGCAGGTGGTGCTGACCGCCCCGGCGGTCTTCGGATTCTTGCGGATTGCCACCAGCC
CCCGGGTCATGGTGTCACCGCTGCCGGTCGCGGACGCCGTCGGATATATACAGTCGTGGCTCGAGCGGCCCAACGTCGAG
TTTCGTTCACCGGGGCCGCGCCACCTGGCGATCGCGTTCGATCTGCTGGCCCGGATCGGGACCGCCGGAAACCTGACCAC
CGACGTGCAACTTGCGGCGTACGCGATCGAGTTCGACGCCGAGATCTGCTCCAACGACGCGGACTTCGGGCGTTTCCCGG
ACGTGCGCTGGGCGAACCCGCTAAGTCCCGGTTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT160421 NZ_CP054782:c132734-132675 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin