Detailed information of TA system TA160385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 41514..42241
• Replicon: plasmid pKP20194a2-p1
• Accession: NZ_CP054775
• Organism: Klebsiella pneumoniae strain KP20194a2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HUR99_RS27060
• Protein ID: WP_011251285.1
• Coordinates: 41930..42241 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUR99_RS27055
• Protein ID: WP_011251286.1
• Coordinates: 41514..41933 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR99_RS27025	36682..37152	-	471	WP_048333570.1	hypothetical protein	-
HUR99_RS27030	37345..38100	-	756	Protein_38	hypothetical protein	-
HUR99_RS27035	38696..39139	+	444	WP_048333569.1	hypothetical protein	-
HUR99_RS27040	39160..39948	+	789	WP_040217257.1	hypothetical protein	-
HUR99_RS27045	39962..40297	+	336	Protein_41	conjugal transfer protein TraI	-
HUR99_RS27050	40399..41367	-	969	WP_074428168.1	IS5 family transposase	-
HUR99_RS27055	41514..41933	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HUR99_RS27060	41930..42241	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUR99_RS27065	42446..42904	-	459	Protein_45	DDE-type integrase/transposase/recombinase	-
HUR99_RS27070	43018..43715	+	698	Protein_46	IS1 family transposase	-
HUR99_RS27075	43731..44450	-	720	Protein_47	aldo/keto reductase	-
HUR99_RS27080	44447..44926	-	480	WP_004212799.1	hypothetical protein	-
HUR99_RS27085	44923..45108	-	186	WP_045145446.1	hypothetical protein	-
HUR99_RS27090	45112..45228	+	117	Protein_50	Tn3 family transposase	-
HUR99_RS27095	45511..45741	-	231	WP_011154571.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195030	195030
	-	inside	IScluster/Tn	-	-	22688..43715	21027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T160385 WP_011251285.1 NZ_CP054775:c42241-41930 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T160385 NZ_CP070482:c2911226-2910876 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT160385 WP_011251286.1 NZ_CP054775:c41933-41514 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT160385 NZ_CP070482:c2911471-2911220 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1