Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
Location | 41517..42244 | Replicon | plasmid pKP20194b-p1 |
Accession | NZ_CP054769 | ||
Organism | Klebsiella pneumoniae strain KP20194b |
Toxin (Protein)
Gene name | higB | Uniprot ID | A0A7W3D9W1 |
Locus tag | HUS00_RS27060 | Protein ID | WP_011251285.1 |
Coordinates | 41933..42244 (-) | Length | 104 a.a. |
Antitoxin (Protein)
Gene name | higA | Uniprot ID | - |
Locus tag | HUS00_RS27055 | Protein ID | WP_011251286.1 |
Coordinates | 41517..41936 (-) | Length | 140 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
HUS00_RS27025 | 36685..37155 | - | 471 | WP_048333570.1 | hypothetical protein | - |
HUS00_RS27030 | 37348..38103 | - | 756 | Protein_38 | hypothetical protein | - |
HUS00_RS27035 | 38699..39142 | + | 444 | WP_048333569.1 | hypothetical protein | - |
HUS00_RS27040 | 39163..39951 | + | 789 | WP_040217257.1 | hypothetical protein | - |
HUS00_RS27045 | 39965..40300 | + | 336 | Protein_41 | conjugal transfer protein TraI | - |
HUS00_RS27050 | 40402..41370 | - | 969 | WP_074428168.1 | IS5 family transposase | - |
HUS00_RS27055 | 41517..41936 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
HUS00_RS27060 | 41933..42244 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
HUS00_RS27065 | 42449..42907 | - | 459 | Protein_45 | DDE-type integrase/transposase/recombinase | - |
HUS00_RS27070 | 43021..43718 | + | 698 | Protein_46 | IS1 family transposase | - |
HUS00_RS27075 | 43734..44453 | - | 720 | Protein_47 | aldo/keto reductase | - |
HUS00_RS27080 | 44450..44929 | - | 480 | WP_004212799.1 | hypothetical protein | - |
HUS00_RS27085 | 44926..45111 | - | 186 | WP_045145446.1 | hypothetical protein | - |
HUS00_RS27090 | 45115..45231 | + | 117 | Protein_50 | Tn3 family transposase | - |
HUS00_RS27095 | 45514..45744 | - | 231 | WP_011154571.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Mobilizable plasmid | - | iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..195034 | 195034 | |
- | inside | IScluster/Tn | - | - | 22688..43718 | 21030 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T160355 WP_011251285.1 NZ_CP054769:c42244-41933 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T160355 NZ_CP070465:c24068-23745 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT160355 WP_011251286.1 NZ_CP054769:c41936-41517 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT160355 NZ_CP070465:c23696-23394 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|