Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 41517..42244 Replicon plasmid pKP20194b-p1
Accession NZ_CP054769
Organism Klebsiella pneumoniae strain KP20194b

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag HUS00_RS27060 Protein ID WP_011251285.1
Coordinates 41933..42244 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUS00_RS27055 Protein ID WP_011251286.1
Coordinates 41517..41936 (-) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS00_RS27025 36685..37155 - 471 WP_048333570.1 hypothetical protein -
HUS00_RS27030 37348..38103 - 756 Protein_38 hypothetical protein -
HUS00_RS27035 38699..39142 + 444 WP_048333569.1 hypothetical protein -
HUS00_RS27040 39163..39951 + 789 WP_040217257.1 hypothetical protein -
HUS00_RS27045 39965..40300 + 336 Protein_41 conjugal transfer protein TraI -
HUS00_RS27050 40402..41370 - 969 WP_074428168.1 IS5 family transposase -
HUS00_RS27055 41517..41936 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
HUS00_RS27060 41933..42244 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
HUS00_RS27065 42449..42907 - 459 Protein_45 DDE-type integrase/transposase/recombinase -
HUS00_RS27070 43021..43718 + 698 Protein_46 IS1 family transposase -
HUS00_RS27075 43734..44453 - 720 Protein_47 aldo/keto reductase -
HUS00_RS27080 44450..44929 - 480 WP_004212799.1 hypothetical protein -
HUS00_RS27085 44926..45111 - 186 WP_045145446.1 hypothetical protein -
HUS00_RS27090 45115..45231 + 117 Protein_50 Tn3 family transposase -
HUS00_RS27095 45514..45744 - 231 WP_011154571.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..195034 195034
- inside IScluster/Tn - - 22688..43718 21030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T160355 WP_011251285.1 NZ_CP054769:c42244-41933 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T160355 NZ_CP070465:c24068-23745 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT160355 WP_011251286.1 NZ_CP054769:c41936-41517 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT160355 NZ_CP070465:c23696-23394 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References