Detailed information of TA system TA160298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 41509..42236
• Replicon: plasmid pKP20194c-p1
• Accession: NZ_CP054757
• Organism: Klebsiella pneumoniae strain KP20194c

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HUS02_RS27070
• Protein ID: WP_011251285.1
• Coordinates: 41925..42236 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUS02_RS27065
• Protein ID: WP_011251286.1
• Coordinates: 41509..41928 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS02_RS27035	36677..37147	-	471	WP_048333570.1	hypothetical protein	-
HUS02_RS27040	37340..38095	-	756	Protein_38	hypothetical protein	-
HUS02_RS27045	38691..39134	+	444	WP_048333569.1	hypothetical protein	-
HUS02_RS27050	39155..39943	+	789	WP_040217257.1	hypothetical protein	-
HUS02_RS27055	39957..40292	+	336	Protein_41	conjugal transfer protein TraI	-
HUS02_RS27060	40394..41362	-	969	WP_074428168.1	IS5 family transposase	-
HUS02_RS27065	41509..41928	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HUS02_RS27070	41925..42236	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUS02_RS27075	42441..42899	-	459	Protein_45	DDE-type integrase/transposase/recombinase	-
HUS02_RS27080	43013..43710	+	698	Protein_46	IS1 family transposase	-
HUS02_RS27085	43726..44445	-	720	Protein_47	aldo/keto reductase	-
HUS02_RS27090	44442..44921	-	480	WP_004212799.1	hypothetical protein	-
HUS02_RS27095	44918..45103	-	186	WP_045145446.1	hypothetical protein	-
HUS02_RS27100	45107..45223	+	117	Protein_50	Tn3 family transposase	-
HUS02_RS27105	45506..45736	-	231	WP_011154571.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195024	195024
	-	inside	IScluster/Tn	-	-	22688..43710	21022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T160298 WP_011251285.1 NZ_CP054757:c42236-41925 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T160298 NZ_CP070440:824979-825389 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGTAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAACAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATCCCGGAGAGTGGCGGGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT160298 WP_011251286.1 NZ_CP054757:c41928-41509 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT160298 NZ_CP070440:824732-824998 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1