Detailed information of TA system TA160276

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132676..132929
• Replicon: plasmid pKP20194c3-p2
• Accession: NZ_CP054752
• Organism: Klebsiella pneumoniae strain KP20194c3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUS03_RS28765
• Protein ID: WP_001312851.1
• Coordinates: 132780..132929 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	132676..132735 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS03_RS28725	128058..128402	-	345	Protein_157	IS6-like element IS26 family transposase	-
HUS03_RS28730	128454..129158	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HUS03_RS28735	129222..129383	+	162	Protein_159	DNA helicase	-
HUS03_RS28740	129403..130149	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HUS03_RS28745	130204..130764	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HUS03_RS28750	130896..131096	+	201	WP_015059022.1	hypothetical protein	-
HUS03_RS28755	131482..132081	+	600	WP_032083981.1	hypothetical protein	-
HUS03_RS28760	132245..132475	+	231	WP_001736714.1	hypothetical protein	-
-	132676..132735	-	60	NuclAT_1	-	Antitoxin
-	132676..132735	-	60	NuclAT_1	-	Antitoxin
-	132676..132735	-	60	NuclAT_1	-	Antitoxin
-	132676..132735	-	60	NuclAT_1	-	Antitoxin
HUS03_RS28765	132780..132929	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUS03_RS28770	133213..133461	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133772	133772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T160276 WP_001312851.1 NZ_CP054752:132780-132929 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T160276 NZ_CP070430:1926089-1926191 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 60 bp

>AT160276 NZ_CP054752:c132735-132676 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin