Detailed information of TA system TA160273

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35049..35318
• Replicon: plasmid pKP20194c3-p2
• Accession: NZ_CP054752
• Organism: Klebsiella pneumoniae strain KP20194c3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HUS03_RS28165
• Protein ID: WP_001312861.1
• Coordinates: 35160..35318 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35049..35114 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS03_RS28140	30759..31286	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HUS03_RS28145	31344..31577	+	234	WP_000006003.1	DUF905 family protein	-
HUS03_RS28150	31638..33661	+	2024	Protein_42	ParB/RepB/Spo0J family partition protein	-
HUS03_RS28155	33730..34164	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HUS03_RS28160	34161..34880	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	35049..35114	+	66	-	-	Antitoxin
HUS03_RS28165	35160..35318	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUS03_RS28170	35556..35933	-	378	Protein_46	hypothetical protein	-
HUS03_RS28175	36233..36529	+	297	WP_001272251.1	hypothetical protein	-
HUS03_RS28180	36640..37461	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HUS03_RS28185	37758..38405	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HUS03_RS28190	38682..39065	+	384	WP_000124981.1	relaxosome protein TraM	-
HUS03_RS28195	39346..40050	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133772	133772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T160273 WP_001312861.1 NZ_CP054752:35160-35318 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T160273 NZ_CP070425:c5278268-5277885 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT160273 NZ_CP054752:35049-35114 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin