Detailed information of TA system TA160211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 41517..42244
• Replicon: plasmid pKP20194c5-p1
• Accession: NZ_CP054739
• Organism: Klebsiella pneumoniae strain KP20194c5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HUS05_RS27075
• Protein ID: WP_011251285.1
• Coordinates: 41933..42244 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUS05_RS27070
• Protein ID: WP_011251286.1
• Coordinates: 41517..41936 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS05_RS27040	36685..37155	-	471	WP_048333570.1	hypothetical protein	-
HUS05_RS27045	37348..38103	-	756	Protein_38	hypothetical protein	-
HUS05_RS27050	38699..39142	+	444	WP_048333569.1	hypothetical protein	-
HUS05_RS27055	39163..39951	+	789	WP_040217257.1	hypothetical protein	-
HUS05_RS27060	39965..40300	+	336	Protein_41	conjugal transfer protein TraI	-
HUS05_RS27065	40402..41370	-	969	WP_074428168.1	IS5 family transposase	-
HUS05_RS27070	41517..41936	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HUS05_RS27075	41933..42244	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUS05_RS27080	42449..42907	-	459	Protein_45	DDE-type integrase/transposase/recombinase	-
HUS05_RS27085	43021..43718	+	698	Protein_46	IS1 family transposase	-
HUS05_RS27090	43734..44453	-	720	Protein_47	aldo/keto reductase	-
HUS05_RS27095	44450..44929	-	480	WP_004212799.1	hypothetical protein	-
HUS05_RS27100	44926..45111	-	186	WP_045145446.1	hypothetical protein	-
HUS05_RS27105	45115..45231	+	117	Protein_50	Tn3 family transposase	-
HUS05_RS27110	45514..45744	-	231	WP_011154571.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195034	195034
	-	inside	IScluster/Tn	-	-	22688..43718	21030

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T160211 WP_011251285.1 NZ_CP054739:c42244-41933 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T160211 NZ_CP070338:c3804917-3804525 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT160211 WP_011251286.1 NZ_CP054739:c41936-41517 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT160211 NZ_CP070338:c3805192-3804917 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1